Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals.
Since Bacillus cereus is one of the important foodborne pathogens, it is interesting to investigate the biosafety of Bacillus spp. and B. cereus in ready-to-eat cereals marketed local supermarkets. For this investigation, the prevalence and enumeration of Bacillus spp. and B. cereus were assayed usi...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English English English |
Published: |
Elsevier
2009
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Online Access: | http://psasir.upm.edu.my/id/eprint/7742/1/Application%20of%20MPN.pdf http://psasir.upm.edu.my/id/eprint/7742/2/Application%20of%20MPN-PCR%20in%20biosafety%20of%20Bacillus%20cereus%20s.l.%20for%20ready-to-eat%20cereals.pdf http://psasir.upm.edu.my/id/eprint/7742/ http://dx.doi.org/10.1016/j.foodcont.2009.01.009 |
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Summary: | Since Bacillus cereus is one of the important foodborne pathogens, it is interesting to investigate the biosafety of Bacillus spp. and B. cereus in ready-to-eat cereals marketed local supermarkets. For this investigation, the prevalence and enumeration of Bacillus spp. and B. cereus were assayed using MPN-PCR method. Results showed that 78% of the processed cereal products intended for direct consumption were positive for the presence of B. cereus with concentrations ranging from as low as 30 MPN/g to more than 24,000 MPN/g. The concentration obtained from this study also reflects on the differences in the contamination level between the infant food, raw cereals, cereal bars, ready-to-eat breakfast cereals and pre-mixed drinks examined. Hence, application of the MPN-PCR method was found to be useful to address the biosafety concerns of B. cereus in ready-to-eat cereals. |
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