The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation

Solid-state fermentation (SSF), which is an excellent alternative for industrial enzyme production, entails the exploitation of cheap agro-industrial residues (low priced culture media). This paper delves into the cultivation of mould (GRAS Penicillium candium PCA 1/TT031) using wheat bran (WB) as t...

Full description

Saved in:
Bibliographic Details
Main Authors: Alhelli, Amaal M., Abd Manap, Mohd Yazid, Mohammed, Abdulkarim Sabo, Mirhosseini, Seyed Hamed, Sukor, Rashidah, Mohammed, Nameer Khairullah, Khalil, Eilaf Suliman, Meor Hussin, Anis Shobirin
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/77407/1/1%20-%20IFRJ171072.R1%20-%20Revised.pdf
http://psasir.upm.edu.my/id/eprint/77407/
http://www.ifrj.upm.edu.my/25%20(07)%202018%20supplementary%201/1%20-%20IFRJ171072.R1%20-%20Revised.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.77407
record_format eprints
spelling my.upm.eprints.774072020-03-05T06:32:00Z http://psasir.upm.edu.my/id/eprint/77407/ The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation Alhelli, Amaal M. Abd Manap, Mohd Yazid Mohammed, Abdulkarim Sabo Mirhosseini, Seyed Hamed Sukor, Rashidah Mohammed, Nameer Khairullah Khalil, Eilaf Suliman Meor Hussin, Anis Shobirin Solid-state fermentation (SSF), which is an excellent alternative for industrial enzyme production, entails the exploitation of cheap agro-industrial residues (low priced culture media). This paper delves into the cultivation of mould (GRAS Penicillium candium PCA 1/TT031) using wheat bran (WB) as the culture media. The parameters for crude enzymatic extraction of lipase production were optimized to achieve the highest possible lipase activity. An incubation period of 7 days (3.06 U/g WB), 2% of tributyrin (5.43 U/g WB), meat peptone equal to 2% (5.2 U/g WB), moisture content of 50% (v:w) (6.6 U/g WB), initial pH of 9.0( 8.6 U/g WB), inoculum size of 5×106 spore/g of WB (11.3 U/g WB), an incubation temperature of 20°C (13.6 U/g WB), and an extractant consisting of phosphate buffer pH 7.0 (14.7 U/g WB) were the conditions applied for this study. Faculty of Food Science and Technology, Universiti Putra Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77407/1/1%20-%20IFRJ171072.R1%20-%20Revised.pdf Alhelli, Amaal M. and Abd Manap, Mohd Yazid and Mohammed, Abdulkarim Sabo and Mirhosseini, Seyed Hamed and Sukor, Rashidah and Mohammed, Nameer Khairullah and Khalil, Eilaf Suliman and Meor Hussin, Anis Shobirin (2018) The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation. International Food Research Journal, 25 (Suppl. 1). S1-S11. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/25%20(07)%202018%20supplementary%201/1%20-%20IFRJ171072.R1%20-%20Revised.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Solid-state fermentation (SSF), which is an excellent alternative for industrial enzyme production, entails the exploitation of cheap agro-industrial residues (low priced culture media). This paper delves into the cultivation of mould (GRAS Penicillium candium PCA 1/TT031) using wheat bran (WB) as the culture media. The parameters for crude enzymatic extraction of lipase production were optimized to achieve the highest possible lipase activity. An incubation period of 7 days (3.06 U/g WB), 2% of tributyrin (5.43 U/g WB), meat peptone equal to 2% (5.2 U/g WB), moisture content of 50% (v:w) (6.6 U/g WB), initial pH of 9.0( 8.6 U/g WB), inoculum size of 5×106 spore/g of WB (11.3 U/g WB), an incubation temperature of 20°C (13.6 U/g WB), and an extractant consisting of phosphate buffer pH 7.0 (14.7 U/g WB) were the conditions applied for this study.
format Article
author Alhelli, Amaal M.
Abd Manap, Mohd Yazid
Mohammed, Abdulkarim Sabo
Mirhosseini, Seyed Hamed
Sukor, Rashidah
Mohammed, Nameer Khairullah
Khalil, Eilaf Suliman
Meor Hussin, Anis Shobirin
spellingShingle Alhelli, Amaal M.
Abd Manap, Mohd Yazid
Mohammed, Abdulkarim Sabo
Mirhosseini, Seyed Hamed
Sukor, Rashidah
Mohammed, Nameer Khairullah
Khalil, Eilaf Suliman
Meor Hussin, Anis Shobirin
The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation
author_facet Alhelli, Amaal M.
Abd Manap, Mohd Yazid
Mohammed, Abdulkarim Sabo
Mirhosseini, Seyed Hamed
Sukor, Rashidah
Mohammed, Nameer Khairullah
Khalil, Eilaf Suliman
Meor Hussin, Anis Shobirin
author_sort Alhelli, Amaal M.
title The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation
title_short The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation
title_full The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation
title_fullStr The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation
title_full_unstemmed The extraction of crude enzyme of lipase from Penicillium candidum PCA 1/TT031 by way of solid state fermentation
title_sort extraction of crude enzyme of lipase from penicillium candidum pca 1/tt031 by way of solid state fermentation
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/77407/1/1%20-%20IFRJ171072.R1%20-%20Revised.pdf
http://psasir.upm.edu.my/id/eprint/77407/
http://www.ifrj.upm.edu.my/25%20(07)%202018%20supplementary%201/1%20-%20IFRJ171072.R1%20-%20Revised.pdf
_version_ 1662756656808722432
score 13.209306