Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain
Biosurfactants are becoming important alternative to chemical surfactants in almost every sector in the modern industry because of their improved properties compared to their chemical counterparts. Rhamnolipid is among the best known glycolipid type biosurfactants with effective surface proper...
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my.upm.eprints.758342019-11-20T07:06:37Z http://psasir.upm.edu.my/id/eprint/75834/ Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain Nordin, Nordiyana Biosurfactants are becoming important alternative to chemical surfactants in almost every sector in the modern industry because of their improved properties compared to their chemical counterparts. Rhamnolipid is among the best known glycolipid type biosurfactants with effective surface properties and high productivity. This study aims to isolate and characterize isolated bacterial strain from different environment conditions with the capacity to produce rhamnolipid-biosurfactants when grown on blue agar plate (BAP) selective medium. Four strains with high activity on BAP selective medium which suggested its potential as good rhamnolipid producer were screened out using combination of modified drop-collapse test, oil spreading and emulsification index (E24) test. A strain with an outstanding performance and demonstrated good activity in all the above mentioned screening methods were successfully isolated and showed comparable results against control samples such as Triton-X 100 and sodium n-dodecyl sulfate (SDS), the chemical surfactants. Using morphological, Gram staining, Biolog Gen III MicroPlate analysis and 16s rRNA sequence analysis, the strain was identified as Pseudomonas aeruginosa and it was designed as the RS6 strain. P. aeruginosa RS6 produced rhamnolipid optimally at pH 7.0 when supplemented with 2.5% (v/v) palm oil (10.17 g/L) and palm olein (8.65 g/L). It reduced the surface tension ranging from 26 to 28 mN/m and showed emulsifying capability up to 62.0%. High performance liquid chromatography (HPLC) and fourier transform infrared spectroscopy (FT-IR) confirmed that biosurfactants produced by this strain was rhamnolipid in nature. In this study, an efficient and simple protocol to screen out rhamnolipid-biosurfactant producing bacteria was used and this finding will also help to add novel members to the biosurfactants group and expanded current knowledge regarding the diversity and productive capability biosurfactants from a single specific strain. Therefore, the biosurfactants produced by this strain might be useful as an alternative to chemical surfactants for a wide range of potential applications. 2016-02 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/75834/1/FBSB%202016%2046%20-%20IR.pdf Nordin, Nordiyana (2016) Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain. Masters thesis, Universiti Putra Malaysia. Pseudomonas aeruginosa Surfactants |
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Pseudomonas aeruginosa Surfactants Nordin, Nordiyana Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain |
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Biosurfactants are becoming important alternative to chemical surfactants in almost
every sector in the modern industry because of their improved properties compared to
their chemical counterparts. Rhamnolipid is among the best known glycolipid type
biosurfactants with effective surface properties and high productivity. This study aims
to isolate and characterize isolated bacterial strain from different environment
conditions with the capacity to produce rhamnolipid-biosurfactants when grown on
blue agar plate (BAP) selective medium. Four strains with high activity on BAP
selective medium which suggested its potential as good rhamnolipid producer were
screened out using combination of modified drop-collapse test, oil spreading and
emulsification index (E24) test. A strain with an outstanding performance and
demonstrated good activity in all the above mentioned screening methods were
successfully isolated and showed comparable results against control samples such as
Triton-X 100 and sodium n-dodecyl sulfate (SDS), the chemical surfactants. Using
morphological, Gram staining, Biolog Gen III MicroPlate analysis and 16s rRNA
sequence analysis, the strain was identified as Pseudomonas aeruginosa and it was
designed as the RS6 strain. P. aeruginosa RS6 produced rhamnolipid optimally at pH
7.0 when supplemented with 2.5% (v/v) palm oil (10.17 g/L) and palm olein (8.65
g/L). It reduced the surface tension ranging from 26 to 28 mN/m and showed
emulsifying capability up to 62.0%. High performance liquid chromatography
(HPLC) and fourier transform infrared spectroscopy (FT-IR) confirmed that
biosurfactants produced by this strain was rhamnolipid in nature. In this study, an
efficient and simple protocol to screen out rhamnolipid-biosurfactant producing
bacteria was used and this finding will also help to add novel members to the
biosurfactants group and expanded current knowledge regarding the diversity and
productive capability biosurfactants from a single specific strain. Therefore, the
biosurfactants produced by this strain might be useful as an alternative to chemical
surfactants for a wide range of potential applications. |
format |
Thesis |
author |
Nordin, Nordiyana |
author_facet |
Nordin, Nordiyana |
author_sort |
Nordin, Nordiyana |
title |
Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain |
title_short |
Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain |
title_full |
Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain |
title_fullStr |
Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain |
title_full_unstemmed |
Production and characterization of biosurfactant by isolated Pseudomonas aeruginosa RS6 strain |
title_sort |
production and characterization of biosurfactant by isolated pseudomonas aeruginosa rs6 strain |
publishDate |
2016 |
url |
http://psasir.upm.edu.my/id/eprint/75834/1/FBSB%202016%2046%20-%20IR.pdf http://psasir.upm.edu.my/id/eprint/75834/ |
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1651869234731417600 |
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13.160551 |