High-temperature crystallization of thermostable T1 lipase

• Main Authors: Leow, Thean Chor, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran
• Format: Article
• Language: English
• Published: American Chemical Society 2007
• Online Access: http://psasir.upm.edu.my/id/eprint/7524/1/High-Temperature%20Crystallization%20of%20Thermostable%20T1%20Lipase.pdf; http://psasir.upm.edu.my/id/eprint/7524/; http://pubs.acs.org/doi/abs/10.1021/cg050506z

The gene encoding thermostable T1 lipase secreted by Geobacillus sp. strain T1 has been overexpressed in a prokaryotic system. Preliminary crystallization was conducted with crystal screen and crystal screen II through a sitting drop vapor diffusion method with 0.5 mg/mL purified T1 lipase at 16 °C....