Comparison of PCR assay with serum and whole blood samples of experimental trials for detection and differentiation of Brucella melitensis.

• Main Authors: Takele, Belay Y., Bejo, Siti Khairani, Bahaman, Abdul Rani, Omar, Abdul Rahman
• Format: Article
• Language: English; English
• Published: Medwell Online 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/7494/1/Comparison%20of%20PCR%20assay%20with%20serum%20and%20whole%20blood%20samples%20of%20experimental%20trials%20for%20detection%20and%20differentiation%20of%20Brucella%20melitensis.pdf; http://psasir.upm.edu.my/id/eprint/7494/

Brucellosis poses a significant animal and public health problem in many developing countries and requires fast and accurate diagnosis. A PCR assay amplifying part of the Brucella melitensis specific IS711 gene was developed and applied to mice clinical samples on an experimental trial. Over an 8 we...