Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm
This study aims to assess the effect of Eurycoma longifolia aqueous extract on chilled and cryopreserved quality of bull sperm. Semen samples were obtained from four Simmental-Brangus. Each sample was divided into two fractions: the first fraction was used for chilling the semen, and the second frac...
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Elsevier
2018
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Online Access: | http://psasir.upm.edu.my/id/eprint/74565/1/Impact%20of%20Eurycoma%20longifolia%20extract%20on%20DNA%20integrity%2C%20lipid%20peroxidation%2C%20and%20functional%20parameters%20in%20chilled%20and%20cryopreserved%20bull%20sperm.pdf http://psasir.upm.edu.my/id/eprint/74565/ https://www.sciencedirect.com/science/article/pii/S0011224017302985?via%3Dihub |
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my.upm.eprints.745652020-02-06T06:31:38Z http://psasir.upm.edu.my/id/eprint/74565/ Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Jeber, Zaid Khuder Mahmood Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Harighi, Mohammadfazel This study aims to assess the effect of Eurycoma longifolia aqueous extract on chilled and cryopreserved quality of bull sperm. Semen samples were obtained from four Simmental-Brangus. Each sample was divided into two fractions: the first fraction was used for chilling the semen, and the second fraction was used for the freezing process. Both fractions were extended with Tris-egg yolk extender supplemented with 0.0, 0.25, 0.5, 1.0, 2.5, 5.0, and 7.5 mg/ml Eurycoma longifolia aqueous extract. The diluted chilled fraction was chilled at 5 °C for 6 days, whereas the frozen-thawed fraction was frozen in liquid nitrogen. Data revealed that 1 mg/ml E. longifolia aqueous extract yielded significantly (p < .05) higher sperm motility, morphology, viability, and sperm membrane integrity compared with the control group and other treated groups in chilled semen evaluation. For cryopreserved sperm, a significant difference (p < .05) in sperm motility, viability, sperm membrane integrity, DNA integrity, and lipid peroxidation was observed between 5 mg/ml E. longifolia aqueous extract and other treated and control groups. However, no significant difference in the percentage of sperm exhibiting normal sperm morphology was observed among the groups. In conclusion, the addition of 0.25 and 1 mg/ml E. langifolia extract to chilled semen and 5 mg/ml E. longifolia aqueous extract to cryopreserved sperm into Tris-egg yolk extender helps in maintaining superior quality of bull spermatozoa during chilling and freezing. Elsevier 2018-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74565/1/Impact%20of%20Eurycoma%20longifolia%20extract%20on%20DNA%20integrity%2C%20lipid%20peroxidation%2C%20and%20functional%20parameters%20in%20chilled%20and%20cryopreserved%20bull%20sperm.pdf Baiee, Falah Hasan Ali and Haron, Abd Wahid and Yusoff, Rosnina and Omar, Mohamed Ariff and Degu, Nurhusien Yimer and Jeber, Zaid Khuder Mahmood and Ghareeb, Salman Hammadi and Abdelbagi, Tarig Abdelrouf Ahmedeltayeb and Harighi, Mohammadfazel (2018) Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm. Cryobiology, 80. pp. 43-50. ISSN 0011-2240; ESSN: 1090-2392 https://www.sciencedirect.com/science/article/pii/S0011224017302985?via%3Dihub 10.1016/j.cryobiol.2017.12.006 |
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This study aims to assess the effect of Eurycoma longifolia aqueous extract on chilled and cryopreserved quality of bull sperm. Semen samples were obtained from four Simmental-Brangus. Each sample was divided into two fractions: the first fraction was used for chilling the semen, and the second fraction was used for the freezing process. Both fractions were extended with Tris-egg yolk extender supplemented with 0.0, 0.25, 0.5, 1.0, 2.5, 5.0, and 7.5 mg/ml Eurycoma longifolia aqueous extract. The diluted chilled fraction was chilled at 5 °C for 6 days, whereas the frozen-thawed fraction was frozen in liquid nitrogen. Data revealed that 1 mg/ml E. longifolia aqueous extract yielded significantly (p < .05) higher sperm motility, morphology, viability, and sperm membrane integrity compared with the control group and other treated groups in chilled semen evaluation. For cryopreserved sperm, a significant difference (p < .05) in sperm motility, viability, sperm membrane integrity, DNA integrity, and lipid peroxidation was observed between 5 mg/ml E. longifolia aqueous extract and other treated and control groups. However, no significant difference in the percentage of sperm exhibiting normal sperm morphology was observed among the groups. In conclusion, the addition of 0.25 and 1 mg/ml E. langifolia extract to chilled semen and 5 mg/ml E. longifolia aqueous extract to cryopreserved sperm into Tris-egg yolk extender helps in maintaining superior quality of bull spermatozoa during chilling and freezing. |
format |
Article |
author |
Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Jeber, Zaid Khuder Mahmood Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Harighi, Mohammadfazel |
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Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Jeber, Zaid Khuder Mahmood Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Harighi, Mohammadfazel Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm |
author_facet |
Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Jeber, Zaid Khuder Mahmood Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Harighi, Mohammadfazel |
author_sort |
Baiee, Falah Hasan Ali |
title |
Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm |
title_short |
Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm |
title_full |
Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm |
title_fullStr |
Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm |
title_full_unstemmed |
Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm |
title_sort |
impact of eurycoma longifolia extract on dna integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://psasir.upm.edu.my/id/eprint/74565/1/Impact%20of%20Eurycoma%20longifolia%20extract%20on%20DNA%20integrity%2C%20lipid%20peroxidation%2C%20and%20functional%20parameters%20in%20chilled%20and%20cryopreserved%20bull%20sperm.pdf http://psasir.upm.edu.my/id/eprint/74565/ https://www.sciencedirect.com/science/article/pii/S0011224017302985?via%3Dihub |
_version_ |
1662756493860012032 |
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13.18916 |