Cover Image

A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.

The surface antigen (HBsAg) of hepatitis B virus (HBV) is conformational and generally evokes protective humoral immune response in human. A disulfide constrained, andom heptapeptide library displayed on the coat protein III of filamentous bacteriophage M13 was employed to select specific ligands th...

Full description

Saved in:
Bibliographic Details
Main Authors: Wen, Siang Tan, Geok, Hun Tan, Yusoff, Khatijah, Heng, Fong Seow
Format: Article
Language:English
English
Published: Elsevier 2005
Online Access:http://psasir.upm.edu.my/id/eprint/724/1/A%20Phage%20Displayed%20Cyclic%20Peptide%20That%20Interacts%20Tightly%20With%20the%20Immunodominant%20Region%20of%20Hepatitis%20B%20Surface%20Antigen..pdf
http://psasir.upm.edu.my/id/eprint/724/
http://dx.doi.org/10.1016/j.jcv.2005.01.007
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.724
record_format eprints
spelling my.upm.eprints.7242015-10-22T02:43:05Z http://psasir.upm.edu.my/id/eprint/724/ A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. Wen, Siang Tan Geok, Hun Tan Yusoff, Khatijah Heng, Fong Seow The surface antigen (HBsAg) of hepatitis B virus (HBV) is conformational and generally evokes protective humoral immune response in human. A disulfide constrained, andom heptapeptide library displayed on the coat protein III of filamentous bacteriophage M13 was employed to select specific ligands that interact with HBsAg subtype ad. Fusion phages carrying the amino acid sequence ETGAKPH and other related sequences were isolated. The binding site of peptide ETGAKPH was located on the immunodominant region of HBsAg. An equilibrium binding assays in solution showed that the phage binds tightly to HBsAg with a relative dissociation constant(krel D) of 2.9 ± 0.9 nM. The phage bearing this peptide has the potential to be used as a diagnostic reagent and two assays for detecting HBsAg in blood samples are described. Elsevier 2005 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/724/1/A%20Phage%20Displayed%20Cyclic%20Peptide%20That%20Interacts%20Tightly%20With%20the%20Immunodominant%20Region%20of%20Hepatitis%20B%20Surface%20Antigen..pdf Wen, Siang Tan and Geok, Hun Tan and Yusoff, Khatijah and Heng, Fong Seow (2005) A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen. Journal of Clinical Virology, 34 (1). pp. 35-41. ISSN 1386-6532 http://dx.doi.org/10.1016/j.jcv.2005.01.007 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The surface antigen (HBsAg) of hepatitis B virus (HBV) is conformational and generally evokes protective humoral immune response in human. A disulfide constrained, andom heptapeptide library displayed on the coat protein III of filamentous bacteriophage M13 was employed to select specific ligands that interact with HBsAg subtype ad. Fusion phages carrying the amino acid sequence ETGAKPH and other related sequences were isolated. The binding site of peptide ETGAKPH was located on the immunodominant region of HBsAg. An equilibrium binding assays in solution showed that the phage binds tightly to HBsAg with a relative dissociation constant(krel D) of 2.9 ± 0.9 nM. The phage bearing this peptide has the potential to be used as a diagnostic reagent and two assays for detecting HBsAg in blood samples are described.
format Article
author Wen, Siang Tan
Geok, Hun Tan
Yusoff, Khatijah
Heng, Fong Seow
spellingShingle Wen, Siang Tan
Geok, Hun Tan
Yusoff, Khatijah
Heng, Fong Seow
A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.
author_facet Wen, Siang Tan
Geok, Hun Tan
Yusoff, Khatijah
Heng, Fong Seow
author_sort Wen, Siang Tan
title A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.
title_short A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.
title_full A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.
title_fullStr A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.
title_full_unstemmed A Phage – Displayed Cyclic Peptide That Interacts Tightly With the Immunodominant Region of Hepatitis B Surface Antigen.
title_sort phage – displayed cyclic peptide that interacts tightly with the immunodominant region of hepatitis b surface antigen.
publisher Elsevier
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/724/1/A%20Phage%20Displayed%20Cyclic%20Peptide%20That%20Interacts%20Tightly%20With%20the%20Immunodominant%20Region%20of%20Hepatitis%20B%20Surface%20Antigen..pdf
http://psasir.upm.edu.my/id/eprint/724/
http://dx.doi.org/10.1016/j.jcv.2005.01.007
_version_ 1643821899822661632
score 13.188404

Similar Items