Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein
Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring th...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/72252/1/Development%20of%20an%20optical%20sensor%20based%20on%20surface%20plasmon%20resonance%20phenomenon%20for%20diagnosis%20of%20dengue%20virus%20E-protein.pdf http://psasir.upm.edu.my/id/eprint/72252/ https://www.sciencedirect.com/science/article/pii/S2214180417301757 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001 nM to 10 nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01 nM, with sensitivity of 39.96° nM−1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface. |
|---|