Construction of IRES-incorporated pMG36e lactococcal vector and its segregational instability in Escherichia coli

• Main Authors: Lim, Y. Y., Ibrahim, Noor Hidayah, Mustapha, Nurul Asyifah
• Format: Article
• Language: English
• Published: Veterinary Association of Malaysia 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/72111/1/Construction%20of%20IRES-incorporated%20pMG36e%20lactococcal%20vector.pdf; http://psasir.upm.edu.my/id/eprint/72111/; http://jvm.vam.org.my/1104-2/

The increasing demand of Lactococcus lactis (L. lactis) beyond application in food industry raises the need to develop lactococcal bicistronic vector with target gene expression in a eukaryotic system for vaccination purposes. Despite the importance of functional studies of the expression vector in...