Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis

• Main Authors: Yuli, Haryani, Awang Saleh, Noorzaleha, Abu Bakar, Fatimah, Mohamed Alitheen, Noorjahan Banu, Patrick, Guda Benjamin, Abdul Talib, Shamsinar, Ahmad Suhaimi, Laila Rabaah, Radu, Son
• Format: Article
• Language: English; English
• Published: Elsevier 2007
• Online Access: http://psasir.upm.edu.my/id/eprint/7155/1/Incidence%20of%20Klebsiella%20pneumonia%20in%20street%20foods%20sold%20in%20Malaysia%20and%20their%20characterization%20by%20antibiotic%20resistance.pdf; http://psasir.upm.edu.my/id/eprint/7155/; http://dx.doi.org/10.1016/j.foodcont.2006.04.009

A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility testing showed that all isolates were highly resistant to ampicillin (100%), erythromycin (100%), rifampicin (100%), streptomycin (96%) and sulfamethoxazole (80%), but susceptible to chloramphenicol. RAPD proved to be the most effective technique in discriminating the K. pneumonia isolates since dendrogram constructed using the combination of 4 primers could differentiate 25 isolates of K. pneumonia to 25 strains. This is the first report that revealed the occurrence of K. pneumonia in street foods sold in Malaysia.