Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K
The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7075/1/Physical%20factors%20affecting%20the%20production%20of%20organic%20solvent.pdf http://psasir.upm.edu.my/id/eprint/7075/ http://www.sciencedirect.com/science/article/pii/S0960852404002238 |
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| Summary: | The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media. |
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