Effect of intranasal attenuated Pasteurella multocida B:2 on haemorrhagic septicaemia in calves

• Main Authors: Khin, Myat Nwe, Saad, Mohd Zamri, Mohamed Mustapha, Noordin, Abd Wahid, Mohd Effendy
• Format: Article
• Language: English
• Published: Online Journal of Veterinary Research 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/7064/1/Effect%20of%20intranasal%20attenuated%20Pasteurella%20multocida%20B.pdf; http://psasir.upm.edu.my/id/eprint/7064/; http://onljvetres.com/pasteurellaabs2009.htm

This report describes the effect of vaccination with attenuated P. multocida B:2 on wild-type P. multocida B:2 infection in calves. Calves were given 5 ml intranasal 107 CFU/mL live attenuated gdhA derivative of P. multocida B:2. Untreated calves were then mingled with the vaccinated Group. Control untreated calves were kept separate. After 6 weeks, all calves were challenged intra-tracheally with 107 CFU/mL of live wild-type P. multocida B:2. At Post-Mortem none of the vaccinated or mingled calves had lesions whereas controls revealed pulmonary petechial haemorrhages with acute pneumonia patches with neutrophils. Both exposed and commingled calves showed significantly (p<0.05) higher levels of serum IgG. P. multocida B:2 were successfully isolated only from the control calves of Group 3. These findings suggest that intranasal exposures to live attenuated P. multocida B:2 prevented infection by wild-type P. multocida B:2 and may have protected susceptible calves.