Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species
Over the recent years, marine sponges have been the target of research as a source of new chemicals with therapeutic potential. They have been proven to have a high strike rate especially for cytotoxic compounds. Thirteen species of marine sponges collected from Pulau Kapas, Pulau Bidong and Pula...
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my.upm.eprints.67812023-11-08T08:12:08Z http://psasir.upm.edu.my/id/eprint/6781/ Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species Kee, Cheng Ling Over the recent years, marine sponges have been the target of research as a source of new chemicals with therapeutic potential. They have been proven to have a high strike rate especially for cytotoxic compounds. Thirteen species of marine sponges collected from Pulau Kapas, Pulau Bidong and Pulau Redang were preliminarily identified as Aaptos sp. (I), Xestospongia exigua (2), unidentified sp. (3), Xestospongia sp. (4), Xestospongia testudinaria (5), Callyspongia sp. (6), Theonella sp. (7), Theonella sp. (S), Sigmaducza arnbolnenuris (9), Ircinia sp. (lo), Dysedza sp. (1 I), unidentified sp. (12) and Ircinia Halisarca (13). The crude methanol extracts of these samples were screened for cytotoxic activities against a panel of cell lines, namely HL-60 (promyelocytic leukemia), CEM-SS (T-lymphoblastic leukemia), MCF-7 (breast cancer), HeLa (cervical cancer), HT-29 (colon cancer) and L929 (murine fibrosarcoma from mouse) using a colorimetric tetrmlium (MTT) assay. Crude extracts from 1 and 10 were active against all six cell lines with CDm values ranging from 1.05 to 24.1 pglml whereas extracts 2, 3 and 8 showed activity only against H1L-60, CEM-SS and HT-29 with CDso values ranpg from 12.95 to 29.5 pglml. Aaptos sp. (1) was chosen for further investigations due to its abundance and strong cytotoxic activity. Bioassay guided isolation and purification of compounds afforded three cytotoxic alkaloids. H19 was identified as the previously isolated aaptamine [I] and the two orange compounds were established as the new aaptaminoid alkaloids, 01 and 02. All three compounds exhibited significant cytotoxic activity against CEM-SS cells with respective CD50 values of 15.0,5.3 and 6.7 pg/ml. When tested against 3T3 (normal mouse fibroblast), all three compounds displayed weak cytotoxicities. The CDS0 of compound 01 and 02 were determined as 21.2 and 21.0 pg/ml respectively. On the other hand, compound 3319 did not achieve a CDSo. Phase contrast microscopic analysis showed that compound H19, 01 and 02 induced apoptosis in CEM-SS cells. The apoptotic features observed include cell shrinkage, condensation of chromatin material, membrane blebbing and the formation of apoptotic bodies. Due to insufficient quantity of 0 1 and 02, only H19 was subjected to subsequent evaluation using fluorescence microscopy. These results further supported that H19 induce apoptosis in CEM-SS as exemplified by the morphological changes observed. 2006-03 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/6781/1/IB_2006_13.pdf Kee, Cheng Ling (2006) Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species. Masters thesis, Universiti Putra Malaysia. Marine biology English |
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Marine biology Kee, Cheng Ling Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species |
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Over the recent years, marine sponges have been the target of research as a source of
new chemicals with therapeutic potential. They have been proven to have a high
strike rate especially for cytotoxic compounds. Thirteen species of marine sponges
collected from Pulau Kapas, Pulau Bidong and Pulau Redang were preliminarily
identified as Aaptos sp. (I), Xestospongia exigua (2), unidentified sp. (3),
Xestospongia sp. (4), Xestospongia testudinaria (5), Callyspongia sp. (6), Theonella
sp. (7), Theonella sp. (S), Sigmaducza arnbolnenuris (9), Ircinia sp. (lo), Dysedza sp.
(1 I), unidentified sp. (12) and Ircinia Halisarca (13). The crude methanol extracts
of these samples were screened for cytotoxic activities against a panel of cell lines,
namely HL-60 (promyelocytic leukemia), CEM-SS (T-lymphoblastic leukemia),
MCF-7 (breast cancer), HeLa (cervical cancer), HT-29 (colon cancer) and L929
(murine fibrosarcoma from mouse) using a colorimetric tetrmlium (MTT) assay.
Crude extracts from 1 and 10 were active against all six cell lines with CDm values
ranging from 1.05 to 24.1 pglml whereas extracts 2, 3 and 8 showed activity only
against H1L-60, CEM-SS and HT-29 with CDso values ranpg from 12.95 to 29.5 pglml. Aaptos sp. (1) was chosen for further investigations due to its abundance and
strong cytotoxic activity. Bioassay guided isolation and purification of compounds
afforded three cytotoxic alkaloids. H19 was identified as the previously isolated
aaptamine [I] and the two orange compounds were established as the new
aaptaminoid alkaloids, 01 and 02. All three compounds exhibited significant
cytotoxic activity against CEM-SS cells with respective CD50 values of 15.0,5.3 and
6.7 pg/ml. When tested against 3T3 (normal mouse fibroblast), all three compounds
displayed weak cytotoxicities. The CDS0 of compound 01 and 02 were determined
as 21.2 and 21.0 pg/ml respectively. On the other hand, compound 3319 did not
achieve a CDSo. Phase contrast microscopic analysis showed that compound H19,
01 and 02 induced apoptosis in CEM-SS cells. The apoptotic features observed
include cell shrinkage, condensation of chromatin material, membrane blebbing and
the formation of apoptotic bodies. Due to insufficient quantity of 0 1 and 02, only
H19 was subjected to subsequent evaluation using fluorescence microscopy. These
results further supported that H19 induce apoptosis in CEM-SS as exemplified by the
morphological changes observed. |
format |
Thesis |
author |
Kee, Cheng Ling |
author_facet |
Kee, Cheng Ling |
author_sort |
Kee, Cheng Ling |
title |
Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species |
title_short |
Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species |
title_full |
Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species |
title_fullStr |
Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species |
title_full_unstemmed |
Screening and Isolation of Cytotoxic Compounds From Local Marine Aaptos Species |
title_sort |
screening and isolation of cytotoxic compounds from local marine aaptos species |
publishDate |
2006 |
url |
http://psasir.upm.edu.my/id/eprint/6781/1/IB_2006_13.pdf http://psasir.upm.edu.my/id/eprint/6781/ |
_version_ |
1783879892479246336 |
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13.18916 |