Prevalence of avian polyomavirus in psittacine birds in the Klang Valley

• Main Authors: Zanon, Zamir, Zainal Abidin, Nurulhuda Najihah, Abu, Jalila, Abdul Razak, Mariatulqabtiah
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia Press 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/64496/1/32%20JTAS%20Vol%2041%20%282%29%20May%202018_JTAS-1179-2017_pg917-924.pdf; http://psasir.upm.edu.my/id/eprint/64496/; http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2041%20(2)%20May.%202018/32%20JTAS%20Vol%2041%20(2)%20May%202018_JTAS-1179-2017_pg917-924.pdf

Avian polyomavirus (APV) primarily affects young birds and can cause mortality in a wide range of psittacine birds. This is the first study to detect the presence of APV in Malaysia. A total of 85 faecal samples were collected from symptom-free psittacines species from four different breeders in the Klang Valley. Upon genomic DNA extraction, the presence of APV was analysed by PCR using primers APVfull-AF (5'- ACAATGCCTAACGGAACGCC-3') and APVfull-AR (5'-CACCGAAGCGGCGATACTATA-3'). Positive results of 3 kbp PCR amplicon were detected in six out of the 30 samples (20%), which were from yellow-collared macaws, blue-headed parrots, red-crowned macaws, sulphur-crested cockatoos, blue-throated macaws, and Pesquet's parrots. As a conclusion, the prevalence of APV was clearly indicated.