The CRISPR growth spurt: from bench to clinic on versatile small RNAs

• Main Authors: Bayat, Hadi, Omidi, Meisam, Masoumeh, Rajabibazl, Rahimpour, Azam, Sabri, Suriana
• Format: Article
• Language: English
• Published: Korean Society for Microbiology and Biotechnology 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/63418/1/The%20CRISPR%20growth%20spurt%20from%20bench%20to%20clinic%20on%20versatile%20small%20RNAs.pdf; http://psasir.upm.edu.my/id/eprint/63418/; http://www.jmb.or.kr/

Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) in association with CRISPR associated protein (Cas) is an adaptive immune system, playing a pivotal role in the defense of bacteria and archaea. Ease of handling and cost effectiveness make CRISPR-Cas system an ideal programmable nuclease tool. Recent advances in understanding the CRISPR-Cas system have tremendously improved its efficiency. For instance, it is possible to recapitulate the chronicle CRISPR-Cas from its infancy and inaugurate a developed version by generating novel variants of Cas proteins, subduing off-target effects and optimization of innovative strategies. In summary, CRISPR-Cas system could be employed in a number of applications including providing model systems, rectification of detrimental mutations, and antiviral therapies.