Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system

• Main Authors: Hadi, Sofijan, Tan, Joo Shun, Abbasiliasi, Sahar, Mustafa, Shuhaimi, Puspaningsih, Nyoman Tri, Kadkhodaei, Saeid, Ariff, Arbakariya
• Format: Article
• Published: Taylor & Francis 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/62923/; https://www.tandfonline.com/doi/full/10.1080/01496395.2016.1268159

A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL)...