Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds

• Main Authors: Rajeendran, Allegendran, Nulit, Rosimah, Yong, Christina Seok Yien, Ibrahim, Mohd. Hafiz, Kalhori, Nahid
• Format: Article
• Language: English
• Published: SCIENCEDOMAIN international 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/62237/1/Isolation%20and%20molecular%20identification%20of%20Colletotrichum%20gleosporiodes.pdf; http://psasir.upm.edu.my/id/eprint/62237/; http://www.journalijpss.com/index.php/IJPSS/issue/view/692

Peanut (Arachis hypogaea(L) cv. Margenta) seeds are currently, significantly considered as an important source of protein and vegetable oil. Anthracnose caused by Colletotrichum gloeosporioidesis a fungal disease from seed that reduces seedling emergence and seed germination. The pathogenic fungi that isolated from peanut were identified using ITS 1and ITS 4 primer with polymerase chain reaction and NCBI database using BlastN algorithm. From NCBI using blastN analysed was 95% identify with other Colletotrichumspecies in NCBI database search. The pathogenicity of Colletotrichum gloeosporioideson peanut seeds and seedlings at 14 days after sowing result was inoculated with pathogenic fungi with 32.3% and control was 83%. Hence, this study was experimented with the object to reveal seed-borne infections on tropical peanut in Malaysia.