Construction of new genetic tools as alternatives for protein overexpression in Escherichia coli and pseudomonas aeruginosa

• Main Authors: Wong, Chee Fah, Raja Abd. Rahman, Raja Noor Zaliha, Basri, Mahiran, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: National Institute of Genetic Engineering and Biotechnology 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/61210/1/Construction%20of%20new%20genetic%20tools%20as%20alternatives%20for%20protein%20overexpression%20in%20Escherichia%20coli%20and%20pseudomonas%20aeruginosa.pdf; http://psasir.upm.edu.my/id/eprint/61210/; http://www.ijbiotech.com/article_64935_5e2328fa7d3233d15c267587e483e931.pdf

Background: Pseudomonas protein expression in E. coli is known to be a setback due to signifi cant genetic variation and absence of several genetic elements in E. coli for regulation and activation of Pseudomonas proteins. Modifi cations in promoter/repressor system and shuttle plasmid maintenance h...