Assessment of three different endometrial cytological sampling methods in postpartum beef cows
The aim of this study was to assess three different cytological endometrial sampling methods used to estimate polymorphonuclear leukocytes (PMN) under high power field (HPF) microscopy and to determine subclinical endometritis in postpartum beef cows. Forty beef cows aged 3-7 years were sampled at w...
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my.upm.eprints.609512019-03-21T02:20:51Z http://psasir.upm.edu.my/id/eprint/60951/ Assessment of three different endometrial cytological sampling methods in postpartum beef cows Mohammed, Salah Noori Degu, Nurhusien Yimer Haron, Abd Wahid Yusoff, Rosnina Mada, Khumran Armiya'u Ebrahimi, Mahdi Baiee, Falah Hasan Ali The aim of this study was to assess three different cytological endometrial sampling methods used to estimate polymorphonuclear leukocytes (PMN) under high power field (HPF) microscopy and to determine subclinical endometritis in postpartum beef cows. Forty beef cows aged 3-7 years were sampled at week three and four after calving by endometrial cytology methods. The cytological sampling methods used included; cotton swab (CS), cytobrush (CB) technique, and low volume flush (LVF), respectively. The mean PMN counts at the third week was higher (p<0.01) (12.2 cells HPF-1 than on the fourth week (4 cells HPF-1). The average PMN counts using CB alone was significantly higher (11.3 cells HPF-1) than CS (7 cells HPF-1) and LVF ( 6 cells HPF-1) methods. Smears from CB had more endometrial cells (58.55 cells HPF-1) at HPF, which was significantly higher (p<0.01) than CS and LVF methods. Both CB and CS methods yielded more intact cells (62.4 % and 61.9 %) (p <0.01) than LVF (52.4 %). The prevalence of subclinical endometritis in the beef cows between 22 and 28 days postpartum using a threshold value of ≥8 % by cytobrush method was 12.5%, which is considered low. In conclusion, CB method was found to be better and effective technique in comparison to other cytological methods used in obtaining endometrial cytology samples. 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60951/1/Assessment%20of%20three%20different%20endometrial%20cytological%20sampling%20methods%20in%20postpartum%20beef%20cows.pdf Mohammed, Salah Noori and Degu, Nurhusien Yimer and Haron, Abd Wahid and Yusoff, Rosnina and Mada, Khumran Armiya'u and Ebrahimi, Mahdi and Baiee, Falah Hasan Ali (2017) Assessment of three different endometrial cytological sampling methods in postpartum beef cows. Journal of Animal and Plant Sciences, 27 (5). pp. 1515-1521. ISSN 1018-7081 |
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The aim of this study was to assess three different cytological endometrial sampling methods used to estimate polymorphonuclear leukocytes (PMN) under high power field (HPF) microscopy and to determine subclinical endometritis in postpartum beef cows. Forty beef cows aged 3-7 years were sampled at week three and four after calving by endometrial cytology methods. The cytological sampling methods used included; cotton swab (CS), cytobrush (CB) technique, and low volume flush (LVF), respectively. The mean PMN counts at the third week was higher (p<0.01) (12.2 cells HPF-1 than on the fourth week (4 cells HPF-1). The average PMN counts using CB alone was significantly higher (11.3 cells HPF-1) than CS (7 cells HPF-1) and LVF ( 6 cells HPF-1) methods. Smears from CB had more endometrial cells (58.55 cells HPF-1) at HPF, which was significantly higher (p<0.01) than CS and LVF methods. Both CB and CS methods yielded more intact cells (62.4 % and 61.9 %) (p <0.01) than LVF (52.4 %). The prevalence of subclinical endometritis in the beef cows between 22 and 28 days postpartum using a threshold value of ≥8 % by cytobrush method was 12.5%, which is considered low. In conclusion, CB method was found to be better and effective technique in comparison to other cytological methods used in obtaining endometrial cytology samples. |
format |
Article |
author |
Mohammed, Salah Noori Degu, Nurhusien Yimer Haron, Abd Wahid Yusoff, Rosnina Mada, Khumran Armiya'u Ebrahimi, Mahdi Baiee, Falah Hasan Ali |
spellingShingle |
Mohammed, Salah Noori Degu, Nurhusien Yimer Haron, Abd Wahid Yusoff, Rosnina Mada, Khumran Armiya'u Ebrahimi, Mahdi Baiee, Falah Hasan Ali Assessment of three different endometrial cytological sampling methods in postpartum beef cows |
author_facet |
Mohammed, Salah Noori Degu, Nurhusien Yimer Haron, Abd Wahid Yusoff, Rosnina Mada, Khumran Armiya'u Ebrahimi, Mahdi Baiee, Falah Hasan Ali |
author_sort |
Mohammed, Salah Noori |
title |
Assessment of three different endometrial cytological sampling methods in postpartum beef cows |
title_short |
Assessment of three different endometrial cytological sampling methods in postpartum beef cows |
title_full |
Assessment of three different endometrial cytological sampling methods in postpartum beef cows |
title_fullStr |
Assessment of three different endometrial cytological sampling methods in postpartum beef cows |
title_full_unstemmed |
Assessment of three different endometrial cytological sampling methods in postpartum beef cows |
title_sort |
assessment of three different endometrial cytological sampling methods in postpartum beef cows |
publishDate |
2017 |
url |
http://psasir.upm.edu.my/id/eprint/60951/1/Assessment%20of%20three%20different%20endometrial%20cytological%20sampling%20methods%20in%20postpartum%20beef%20cows.pdf http://psasir.upm.edu.my/id/eprint/60951/ |
_version_ |
1643837471865176064 |
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13.160551 |