Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests
Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISA...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis
2015
|
Online Access: | http://psasir.upm.edu.my/id/eprint/60503/1/Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%27s%20nests.pdf http://psasir.upm.edu.my/id/eprint/60503/ https://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605?src=recsys&journalCode=tfac20 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.upm.eprints.60503 |
---|---|
record_format |
eprints |
spelling |
my.upm.eprints.605032018-06-07T06:24:21Z http://psasir.upm.edu.my/id/eprint/60503/ Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry. Taylor & Francis 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60503/1/Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%27s%20nests.pdf Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2015) Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests. Food Additives & Contaminants: Part A, 32 (7). pp. 1023-1028. ISSN 1944-0049; ESSN: 1944-0057 https://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605?src=recsys&journalCode=tfac20 10.1080/19440049.2015.1039605 |
institution |
Universiti Putra Malaysia |
building |
UPM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Putra Malaysia |
content_source |
UPM Institutional Repository |
url_provider |
http://psasir.upm.edu.my/ |
language |
English |
description |
Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry. |
format |
Article |
author |
Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
spellingShingle |
Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
author_facet |
Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
author_sort |
Tukiran, Nur Azira |
title |
Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
title_short |
Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
title_full |
Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
title_fullStr |
Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
title_full_unstemmed |
Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
title_sort |
enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
publisher |
Taylor & Francis |
publishDate |
2015 |
url |
http://psasir.upm.edu.my/id/eprint/60503/1/Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%27s%20nests.pdf http://psasir.upm.edu.my/id/eprint/60503/ https://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605?src=recsys&journalCode=tfac20 |
_version_ |
1643837378167570432 |
score |
13.159267 |