Virus-like particle of Macrobrachium rosenbergii nodavirus produced in Spodoptera frugiperda (Sf9) cells is distinctive from that produced in Escherichia coli

• Main Authors: Kueh, Chare Li, Yong, Chean Yeah, Dezfooli, Seyedehsara Masoomi, Bhassu, Subha, Tan, Soon Guan, Tan, Wen Siang
• Format: Article
• Language: English
• Published: American Institute of Chemical Engineers 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/59658/1/Virus-like%20particle%20of%20Macrobrachium%20rosenbergii%20nodavirus%20produced%20in%20Spodoptera%20frugiperda%20%28Sf9%29%20cells%20is%20distinctive%20from%20that%20produced%20in%20Escherichia%20coli.pdf; http://psasir.upm.edu.my/id/eprint/59658/; http://onlinelibrary.wiley.com/wol1/doi/10.1002/btpr.2409/abstract

Macrobrachium rosenbergii nodavirus (MrNV) is a virus native to giant freshwater prawn. Recombinant MrNV capsid protein has been produced in Escherichia coli, which self-assembled into virus-like particles (VLPs). However, this recombinant protein is unstable, degrading and forming heterogenous VLPs...