Addition of L-tyrosine to improve betalain production in red pitaya callus
The aim of this study is to enhance betalain production in red pitaya (Hylocereus polyrhizus) callus through supplementation of the precursor L-tyrosine. Red-pigmented calli were produced on Murashige and Skoog (MS) basal salt medium supplemented with 2 mg/L naphthaleneacetic acid (NAA), 4 mg/L thid...
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Online Access: | http://psasir.upm.edu.my/id/eprint/58344/1/05%20JTAS%20Vol%2040%20%284%29%20Nov%202017_0837-2016_pg521-532.pdf http://psasir.upm.edu.my/id/eprint/58344/ http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(4)%20Nov.%202017/05%20JTAS%20Vol%2040%20(4)%20Nov%202017_0837-2016_pg521-532.pdf |
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my.upm.eprints.583442018-01-25T09:43:18Z http://psasir.upm.edu.my/id/eprint/58344/ Addition of L-tyrosine to improve betalain production in red pitaya callus Abu Faizal, Nor Fadzliana Sekeli, Rogayah Shaharuddin, Noor Azmi Abdullah, Janna Ong The aim of this study is to enhance betalain production in red pitaya (Hylocereus polyrhizus) callus through supplementation of the precursor L-tyrosine. Red-pigmented calli were produced on Murashige and Skoog (MS) basal salt medium supplemented with 2 mg/L naphthaleneacetic acid (NAA), 4 mg/L thidiazuron (TDZ) and different concentrations of L-tyrosine (20, 40, 60, 80, 100 mg/L). The addition of 20 mg/L L-tyrosine was able to increase betacyanin production by 1.5-fold compared to calli grown on Murashige and Skoog (MS) basal salt medium (MSO). The results also revealed that betalain production (calli cultured with 20 mg/L L-tyrosine) was higher than MS medium with 2 mg/L NAA and 4 mg/L TDZ (without L-tyrosine) up to 1.7-fold. Four compounds, namely betacyanin, betaxanthin and two additional compounds, phenolic and flavonoid compounds, were detected in the red-pigmented calli treated with 20 mg/L L-tyrosine that were not found in the fresh fruit sample. This indicates that the callus system has potential to produce betalain pigment and that red-pigmented calli could be an important antioxidant source. Universiti Putra Malaysia Press 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58344/1/05%20JTAS%20Vol%2040%20%284%29%20Nov%202017_0837-2016_pg521-532.pdf Abu Faizal, Nor Fadzliana and Sekeli, Rogayah and Shaharuddin, Noor Azmi and Abdullah, Janna Ong (2017) Addition of L-tyrosine to improve betalain production in red pitaya callus. Pertanika Journal of Tropical Agricultural Science, 40 (4). pp. 521-532. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(4)%20Nov.%202017/05%20JTAS%20Vol%2040%20(4)%20Nov%202017_0837-2016_pg521-532.pdf |
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The aim of this study is to enhance betalain production in red pitaya (Hylocereus polyrhizus) callus through supplementation of the precursor L-tyrosine. Red-pigmented calli were produced on Murashige and Skoog (MS) basal salt medium supplemented with 2 mg/L naphthaleneacetic acid (NAA), 4 mg/L thidiazuron (TDZ) and different concentrations of L-tyrosine (20, 40, 60, 80, 100 mg/L). The addition of 20 mg/L L-tyrosine was able to increase betacyanin production by 1.5-fold compared to calli grown on Murashige and Skoog (MS) basal salt medium (MSO). The results also revealed that betalain production (calli cultured with 20 mg/L L-tyrosine) was higher than MS medium with 2 mg/L NAA and 4 mg/L TDZ (without L-tyrosine) up to 1.7-fold. Four compounds, namely betacyanin, betaxanthin and two additional compounds, phenolic and flavonoid compounds, were detected in the red-pigmented calli treated with 20 mg/L L-tyrosine that were not found in the fresh fruit sample. This indicates that the callus system has potential to produce betalain pigment and that red-pigmented calli could be an important antioxidant source. |
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Article |
author |
Abu Faizal, Nor Fadzliana Sekeli, Rogayah Shaharuddin, Noor Azmi Abdullah, Janna Ong |
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Abu Faizal, Nor Fadzliana Sekeli, Rogayah Shaharuddin, Noor Azmi Abdullah, Janna Ong Addition of L-tyrosine to improve betalain production in red pitaya callus |
author_facet |
Abu Faizal, Nor Fadzliana Sekeli, Rogayah Shaharuddin, Noor Azmi Abdullah, Janna Ong |
author_sort |
Abu Faizal, Nor Fadzliana |
title |
Addition of L-tyrosine to improve betalain production in red pitaya callus |
title_short |
Addition of L-tyrosine to improve betalain production in red pitaya callus |
title_full |
Addition of L-tyrosine to improve betalain production in red pitaya callus |
title_fullStr |
Addition of L-tyrosine to improve betalain production in red pitaya callus |
title_full_unstemmed |
Addition of L-tyrosine to improve betalain production in red pitaya callus |
title_sort |
addition of l-tyrosine to improve betalain production in red pitaya callus |
publisher |
Universiti Putra Malaysia Press |
publishDate |
2017 |
url |
http://psasir.upm.edu.my/id/eprint/58344/1/05%20JTAS%20Vol%2040%20%284%29%20Nov%202017_0837-2016_pg521-532.pdf http://psasir.upm.edu.my/id/eprint/58344/ http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(4)%20Nov.%202017/05%20JTAS%20Vol%2040%20(4)%20Nov%202017_0837-2016_pg521-532.pdf |
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