Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract
An experiment was conducted with the objective to enhance mucosal immunity against ovalbumin (OVA) by co-administration of OVA with an aqueous extract from the fruit of Solanum torvum (STE). Five groups of female ICR mice aged approximately 8 weeks at the commencement of the experiment were caged in...
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Elsevier
2004
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Online Access: | http://psasir.upm.edu.my/id/eprint/56588/1/Enhancement%20of%20ovalbumin-specific%20IgA%20responses%20via%20oral%20boosting%20with%20antigen%20co-administered%20with%20an%20aqueous%20Solanum%20torvum%20extract.pdf http://psasir.upm.edu.my/id/eprint/56588/ http://www.sciencedirect.com/science/article/pii/S0024320504002681 |
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my.upm.eprints.565882017-08-03T04:54:35Z http://psasir.upm.edu.my/id/eprint/56588/ Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract Israf Ali, Daud Ahmad Lajis, Md. Nordin Somchit, Muhammad Nazrul Sulaiman, Mohd Roslan An experiment was conducted with the objective to enhance mucosal immunity against ovalbumin (OVA) by co-administration of OVA with an aqueous extract from the fruit of Solanum torvum (STE). Five groups of female ICR mice aged approximately 8 weeks at the commencement of the experiment were caged in groups of eight and received various treatments. The treatments included OVA alone, OVA with cholera toxin (CT), and OVA with various doses of STE. Mice were primed intraperitoneally with 500 μg of OVA alone or co-administered with 0.1 μg CT, or with 1 μg STE. All mice were boosted orally via gastric intubation 14 days after priming with 10 mg OVA alone, or co-administered with 10 μg CT or with 10 mg, 1 mg or 0.1 mg STE. One week later all mice were killed and organs obtained for analysis of the immune response. Intestinal, faecal and pulmonary OVA-specific sIgA concentration was significantly increased (p<0.05) in mice that received booster combinations of OVA/CT and OVA with all extract doses (p<0.05). Specific serum IgG titres did not differ significantly between groups. It is concluded that STE can significantly enhance secretory immunity in the intestine to OVA with mucosal homing to the lungs. The adjuvant effect of STE is comparable to that of CT. Elsevier 2004 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56588/1/Enhancement%20of%20ovalbumin-specific%20IgA%20responses%20via%20oral%20boosting%20with%20antigen%20co-administered%20with%20an%20aqueous%20Solanum%20torvum%20extract.pdf Israf Ali, Daud Ahmad and Lajis, Md. Nordin and Somchit, Muhammad Nazrul and Sulaiman, Mohd Roslan (2004) Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract. Life Sciences, 75 (4). pp. 397-406. ISSN 0024-3205; ESSN: 1879-0631 http://www.sciencedirect.com/science/article/pii/S0024320504002681 10.1016/j.lfs.2003.10.038 |
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An experiment was conducted with the objective to enhance mucosal immunity against ovalbumin (OVA) by co-administration of OVA with an aqueous extract from the fruit of Solanum torvum (STE). Five groups of female ICR mice aged approximately 8 weeks at the commencement of the experiment were caged in groups of eight and received various treatments. The treatments included OVA alone, OVA with cholera toxin (CT), and OVA with various doses of STE. Mice were primed intraperitoneally with 500 μg of OVA alone or co-administered with 0.1 μg CT, or with 1 μg STE. All mice were boosted orally via gastric intubation 14 days after priming with 10 mg OVA alone, or co-administered with 10 μg CT or with 10 mg, 1 mg or 0.1 mg STE. One week later all mice were killed and organs obtained for analysis of the immune response. Intestinal, faecal and pulmonary OVA-specific sIgA concentration was significantly increased (p<0.05) in mice that received booster combinations of OVA/CT and OVA with all extract doses (p<0.05). Specific serum IgG titres did not differ significantly between groups. It is concluded that STE can significantly enhance secretory immunity in the intestine to OVA with mucosal homing to the lungs. The adjuvant effect of STE is comparable to that of CT. |
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Article |
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Israf Ali, Daud Ahmad Lajis, Md. Nordin Somchit, Muhammad Nazrul Sulaiman, Mohd Roslan |
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Israf Ali, Daud Ahmad Lajis, Md. Nordin Somchit, Muhammad Nazrul Sulaiman, Mohd Roslan Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract |
author_facet |
Israf Ali, Daud Ahmad Lajis, Md. Nordin Somchit, Muhammad Nazrul Sulaiman, Mohd Roslan |
author_sort |
Israf Ali, Daud Ahmad |
title |
Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract |
title_short |
Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract |
title_full |
Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract |
title_fullStr |
Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract |
title_full_unstemmed |
Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract |
title_sort |
enhancement of ovalbumin-specific iga responses via oral boosting with antigen co-administered with an aqueous solanum torvum extract |
publisher |
Elsevier |
publishDate |
2004 |
url |
http://psasir.upm.edu.my/id/eprint/56588/1/Enhancement%20of%20ovalbumin-specific%20IgA%20responses%20via%20oral%20boosting%20with%20antigen%20co-administered%20with%20an%20aqueous%20Solanum%20torvum%20extract.pdf http://psasir.upm.edu.my/id/eprint/56588/ http://www.sciencedirect.com/science/article/pii/S0024320504002681 |
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1643836235642306560 |
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13.18916 |