Recovery of histidine-tagged nucleocapsid protein of Newcastle disease virus using immobilised metal affinity chromatography

• Main Authors: Tan, Yan Peng, Tau, Chuan Ling, Tan, Wen Siang, Yusoff, Khatijah, Tey, Beng Ti
• Format: Article
• Language: English; English
• Published: Elsevier 2006
• Online Access: http://psasir.upm.edu.my/id/eprint/5608/1/Recovery%20of%20histidine.pdf; http://psasir.upm.edu.my/id/eprint/5608/; http://dx.doi.org/10.1016/j.procbio.2005.11.003

An immobilised metal affinity packed bed adsorption chromatography (IMA-PBAC) for the purification of recombinant nucleocapsid protein (NP) of Newcastle disease virus (NDV) directly from clarified feedstock was developed. The XK 16/20 (i.d. = 16 mm) was used as a packed bed column and Streamline che...