Cytokines (IL 1β and IL 6) responses in non-pregnant does infected with corynebacterium pseudotuberculosis following intradermal route of infection in chronic state

• Main Authors: Abdullah, Faez Firdaus Jesse, Abdul Latif, Nur-Amirah, Lim, Eric Teik Chung, Adamu, Lawan, Sarah, Siti Aimi, Zamri-Saad, Mohd, Haron, Abdul Wahid, Mohd Lila, Mohd Azmi, Zakaria, Zunita
• Format: Article
• Language: English
• Published: Pashupati Foundation India 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/55413/1/Cytokines%20%28IL%201%CE%B2%20and%20IL%206%29%20responses%20in%20non-pregnant%20does%20infected%20with%20.pdf; http://psasir.upm.edu.my/id/eprint/55413/

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA) which commonly affects sheep and goats. The disease remains as a major disease causing economic loss to the small ruminant industries. There is little information related to responses of interleukin-1β and interleukin-6 in the chronic states. This study was designed to determine the serum concentrations of interleukin-1β and interleukin-6 (pg/mL) in non-pregnant does experimentally inoculated with Corynebacterium pseudotuberculosis via intradermal route in chronic form. Eighteen non-pregnant healthy Katjang does aged 2 years old were divided randomly into two groups. The control and the treatment groups consist of nine does each and were kept for 3 months. The control group was inoculated with PBS solution while the treatment group was inoculated intradermally with C. pseudotuberculosis. Serum samples were collected every 3 days (72 hours) for 3 months (2064 hours). The present study showed significant increase in IL-1β (278 ± 19.19 pg/mL) after 1day (24 hours) of post infection (p<0.0001) which decreased sharply (98.31 ± 19.19 pg/mL) after 5 days (120 hours) of post infection (p = 0.9293) and attained a significant concentration (217.43 ± 19.19 pg/mL) after 3 months (2064 hours) of post infection (p<0.0048) in does challenged with C. pseudotuberculosis compared to the control group. In contrast, the concentration of IL-6 increased significantly (p<0.0001) to (56.43 ± 1.98 pg/mL) in 2 months (1392 hours) of post infection and then decreased significantly (p<0.0001) to concentration of (22.18 ± 1.98 pg/mL) in 3 months (2064 hours) compared to the control group. In conclusion, the present study indicate that the importunity of C. pseudotuberculosis is associated with persistently high concentrations of IL-1β and low concentration of IL-6 which, when interpreted, could severely contribute to pathological vicissitudes and injury of organs and tissues in the chronic stage of C. pseudotuberculosis infections.