Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes
Problem statement: Vitrification is replacing conventional slow freezing to cryopreserve gametes and embryos especially for in vitro production of embryo in domestic animal species. However, the results are still not satisfactory. The aim of this experiment was to study the effect of different equil...
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my.upm.eprints.54252020-07-22T02:19:43Z http://psasir.upm.edu.my/id/eprint/5425/ Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes Hajarian, Hadi Haron, Abd Wahid Othman, Abas Mazni Yusoff, Rosnina Daliri, Morteza Dashtizad, Mojtaba Abood Haris, Faizah Yap, Chee Kong Jaffar, Fahrul Firdaus Ann, Fazly Problem statement: Vitrification is replacing conventional slow freezing to cryopreserve gametes and embryos especially for in vitro production of embryo in domestic animal species. However, the results are still not satisfactory. The aim of this experiment was to study the effect of different equilibration temperatures on in vitro viability of immature bovine oocytes after vitrification. Approach: Oocytes were obtained from slaughterhouse ovaries. Only grade one oocytes were used. Oocytes were equilibrated in three different temperatures: 32, 37, or 41°C. Immature oocytes were equilibrated in VS1 (7.5 Ethylene Glycol (EG) + 7.5% DMSO) for 10-12 min and then exposed to VS2 (15% EG + 15%DMSO + 0.5M sucrose) for one min. Thereafter oocytes were loaded on hand-made Cryotop and directly plunged into liquid nitrogen. After warming, oocytes were examined for viability, maturation, cleavage and blastocyst production. Results: Oocytes that were equilibrated at 37°C had significantly higher (p<0.05) viability than 41°C, but there were no significant difference between 37 and 41 with 32°C. Maturation rate in 37°C group was significantly higher compared with other groups. The highest percentage of degenerated and germinal vesicle stage oocytes were obtained from 41°C than 32 and 37°C. Cleavage rate of 37°C group (38.77%) was greater than other groups (30.84 and 28.95% for 32 and 41°C, respectively). The highest blastocyst rate was also produced when oocytes equilibrated at 37°C (6.45%). Conclusion: In conclusion, these results indicated that immature bovine oocytes can be equilibrated successfully at 37°C while higher or lower temperature can significantly decrease their subsequent viability and development. Science Publications 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5425/1/ajavsp.2010.71.75.pdf Hajarian, Hadi and Haron, Abd Wahid and Othman, Abas Mazni and Yusoff, Rosnina and Daliri, Morteza and Dashtizad, Mojtaba and Abood Haris, Faizah and Yap, Chee Kong and Jaffar, Fahrul Firdaus and Ann, Fazly (2010) Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes. American Journal of Animal and Veterinary Sciences, 5 (2). pp. 71-75. ISSN 1557-4555; ESSN: 1557-4563 http://thescipub.com/html/10.3844/ajavsp.2010.71.75 10.3844/ajavsp.2010.71.75 |
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Problem statement: Vitrification is replacing conventional slow freezing to cryopreserve gametes and embryos especially for in vitro production of embryo in domestic animal species. However, the results are still not satisfactory. The aim of this experiment was to study the effect of different equilibration temperatures on in vitro viability of immature bovine oocytes after vitrification. Approach: Oocytes were obtained from slaughterhouse ovaries. Only grade one oocytes were used. Oocytes were equilibrated in three different temperatures: 32, 37, or 41°C. Immature oocytes were equilibrated in VS1 (7.5 Ethylene Glycol (EG) + 7.5% DMSO) for 10-12 min and then exposed to VS2 (15% EG + 15%DMSO + 0.5M sucrose) for one min. Thereafter oocytes were loaded on hand-made Cryotop and directly plunged into liquid nitrogen. After warming, oocytes were examined for viability, maturation, cleavage and blastocyst production. Results: Oocytes that were equilibrated at 37°C had significantly higher (p<0.05) viability than 41°C, but there were no significant difference between 37 and 41 with 32°C. Maturation rate in 37°C group was significantly higher compared with other groups. The highest percentage of degenerated and germinal vesicle stage oocytes were obtained from 41°C than 32 and 37°C. Cleavage rate of 37°C group (38.77%) was greater than other groups (30.84 and 28.95% for 32 and 41°C, respectively). The highest blastocyst rate was also produced when oocytes equilibrated at 37°C (6.45%). Conclusion: In conclusion, these results indicated that immature bovine oocytes can be equilibrated successfully at 37°C while higher or lower temperature can significantly
decrease their subsequent viability and development. |
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author |
Hajarian, Hadi Haron, Abd Wahid Othman, Abas Mazni Yusoff, Rosnina Daliri, Morteza Dashtizad, Mojtaba Abood Haris, Faizah Yap, Chee Kong Jaffar, Fahrul Firdaus Ann, Fazly |
spellingShingle |
Hajarian, Hadi Haron, Abd Wahid Othman, Abas Mazni Yusoff, Rosnina Daliri, Morteza Dashtizad, Mojtaba Abood Haris, Faizah Yap, Chee Kong Jaffar, Fahrul Firdaus Ann, Fazly Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes |
author_facet |
Hajarian, Hadi Haron, Abd Wahid Othman, Abas Mazni Yusoff, Rosnina Daliri, Morteza Dashtizad, Mojtaba Abood Haris, Faizah Yap, Chee Kong Jaffar, Fahrul Firdaus Ann, Fazly |
author_sort |
Hajarian, Hadi |
title |
Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes |
title_short |
Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes |
title_full |
Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes |
title_fullStr |
Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes |
title_full_unstemmed |
Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes |
title_sort |
effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes |
publisher |
Science Publications |
publishDate |
2010 |
url |
http://psasir.upm.edu.my/id/eprint/5425/1/ajavsp.2010.71.75.pdf http://psasir.upm.edu.my/id/eprint/5425/ http://thescipub.com/html/10.3844/ajavsp.2010.71.75 |
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1674067959665917952 |
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13.160551 |