Estimation of uncertainty from method validation data: application to a reverse-phase high-performance liquid chromatography method for the determination of amino acids in gelatin using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate reagent

• Main Authors: Mohd Ismail, Azilawati, Mat Hashim, Dzulkifly, Bakar, Jamilah, Mustafa, Shuhaimi, Ismail, Amin
• Format: Article
• Language: English
• Published: Elsevier 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/54191/1/Estimation%20of%20uncertainty%20from%20method%20validation%20data.pdf; http://psasir.upm.edu.my/id/eprint/54191/; https://www.sciencedirect.com/science/article/pii/S0731708516303843?via%3Dihub

A detailed procedure for estimating uncertainty according to the Laboratory of Government Chemists/Valid Analytical Measurement (LGC/VAM) protocol for determination of 18 amino acids in gelatin is proposed. The expanded uncertainty was estimated using mainly the method validation data (precision and...