Molecular cloning and functional expression of a Δ9-fatty acid desaturase from an antarctic Pseudomonas sp. A3

• Main Authors: Garba, Lawal, Mohamad Ali, Mohd Shukuri, Oslan, Siti Nurbaya, Raja Abdul Rahman, Raja Noor Zaliha
• Format: Article
• Language: English
• Published: Public Library of Science 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/53322/1/Molecular%20Cloning%20and%20Functional%20Expression.pdf; http://psasir.upm.edu.my/id/eprint/53322/; http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0160681

Fatty acid desaturase enzymes play an essential role in the synthesis of unsaturated fatty acids. Pseudomonas sp. A3 was found to produce a large amount of palmitoleic and oleic acids after incubation at low temperatures. Using polymerase Chain Reaction (PCR), a novel Δ9- fatty acid desaturase gene...