Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae

• Main Authors: Azizi, Parisa, Yusop, Mohd Rafii, Mahmood, Maziah, Abdullah, Siti Nor Akmar, Musa, Mohamed Hanafi, Abdul Latif, Muhammad, Sahebi, Mahbod, Ashkani, Sadegh
• Format: Article
• Language: English
• Published: Taylor & Francis 2017
• Online Access: http://psasir.upm.edu.my/id/eprint/52423/1/Evaluation%20of%20RNA%20extraction%20methods%20in%20rice%20and%20their%20application%20in%20expression%20analysis%20of%20resistance%20genes%20against%20Magnaporthe%20oryzae.pdf; http://psasir.upm.edu.my/id/eprint/52423/; http://www.tandfonline.com/doi/abs/10.1080/13102818.2016.1259015

Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods. The quantity, quality and integrity of extracted RNA were con...