The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze
The feasibility of using treated oil palm empty fruit bunch (OPEFB) fibre as a substrate for cellulase production by Chaetomium globosum Kunze was studied using a shaking flask fermentation system. The use of 2-mm chemically untreated OPEFB fibre increased cellulase production by about two times com...
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Elsevier Science
1997
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my.upm.eprints.510362017-05-02T04:39:30Z http://psasir.upm.edu.my/id/eprint/51036/ The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze Md. Shah, Umi Kalsom Ariff, Arbakariya H. S., Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail The feasibility of using treated oil palm empty fruit bunch (OPEFB) fibre as a substrate for cellulase production by Chaetomium globosum Kunze was studied using a shaking flask fermentation system. The use of 2-mm chemically untreated OPEFB fibre increased cellulase production by about two times compared to 10-mm fibre. The effect of the different chemicals (NaOH, HCl, HNO3, EDA and EDTA) on the 2-mm fibre was also investigated. Treatment with these chemicals significantly (P < 0·05) increased the cellulose and reduced the lignin contents. Fermentation using OPEFB fibre treated with HNO3(0·5% v/v) gave the highest cellulase production and this was related to its high cellulose content. Cellulase production increased further when autoclaved (121°C, 15 psi for 5 min), chemically treated OPEFB fibre was used. When autoclaved 2-mm OPEFB fibre treated with HNO3 was used as a substrate, the maximum FPase activity and yield obtained were 0·95 U ml−1 and 120·7 U g−1 cellulose, respectively. The cellulase produced by C. globosum contained a high proportion of β-glucosidase. The ratio of specific activity of β-glucosidase to FPase was about 8. The production of all three major components of cellulase (endoglucanase, cellobiohydrolase and β-glucosidase) using pretreated OPEFB fibre were about three times higher than those obtained in fermentations using pure cellulose (Avicel and carboxymethylcellulose). Elsevier Science 1997 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51036/1/51036.pdf Md. Shah, Umi Kalsom and Ariff, Arbakariya and H. S., Zulkifli and Tong, C. C. and Hassan, Mohd Ali and Abdul Karim, Mohamed Ismail (1997) The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze. Bioresource Technology, 62 (1-2). pp. 1-9. ISSN 0960-8524 http://www.sciencedirect.com/science/article/pii/S0960852497001326# 10.1016/S0960-8524(97)00132-6 |
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The feasibility of using treated oil palm empty fruit bunch (OPEFB) fibre as a substrate for cellulase production by Chaetomium globosum Kunze was studied using a shaking flask fermentation system. The use of 2-mm chemically untreated OPEFB fibre increased cellulase production by about two times compared to 10-mm fibre. The effect of the different chemicals (NaOH, HCl, HNO3, EDA and EDTA) on the 2-mm fibre was also investigated. Treatment with these chemicals significantly (P < 0·05) increased the cellulose and reduced the lignin contents. Fermentation using OPEFB fibre treated with HNO3(0·5% v/v) gave the highest cellulase production and this was related to its high cellulose content. Cellulase production increased further when autoclaved (121°C, 15 psi for 5 min), chemically treated OPEFB fibre was used. When autoclaved 2-mm OPEFB fibre treated with HNO3 was used as a substrate, the maximum FPase activity and yield obtained were 0·95 U ml−1 and 120·7 U g−1 cellulose, respectively. The cellulase produced by C. globosum contained a high proportion of β-glucosidase. The ratio of specific activity of β-glucosidase to FPase was about 8. The production of all three major components of cellulase (endoglucanase, cellobiohydrolase and β-glucosidase) using pretreated OPEFB fibre were about three times higher than those obtained in fermentations using pure cellulose (Avicel and carboxymethylcellulose). |
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Md. Shah, Umi Kalsom Ariff, Arbakariya H. S., Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail |
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Md. Shah, Umi Kalsom Ariff, Arbakariya H. S., Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze |
author_facet |
Md. Shah, Umi Kalsom Ariff, Arbakariya H. S., Zulkifli Tong, C. C. Hassan, Mohd Ali Abdul Karim, Mohamed Ismail |
author_sort |
Md. Shah, Umi Kalsom |
title |
The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze |
title_short |
The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze |
title_full |
The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze |
title_fullStr |
The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze |
title_full_unstemmed |
The treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by Chaetomium globosum Kunze |
title_sort |
treatment of oil palm empty fruit bunch fibre for subsequent use as substrate for cellulase production by chaetomium globosum kunze |
publisher |
Elsevier Science |
publishDate |
1997 |
url |
http://psasir.upm.edu.my/id/eprint/51036/1/51036.pdf http://psasir.upm.edu.my/id/eprint/51036/ http://www.sciencedirect.com/science/article/pii/S0960852497001326# |
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1643834839187587072 |
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13.160551 |