Kinetics and modeling of red pigment fermentation by Monascus purpureus FTC 5391 in 2-litre stirred tank fermenter using glucose as a carbon source

• Main Authors: Abdul Manan, Musaalbakri, Ariff, Arbakariya, Mohamad, Rosfarizan, Abdul Karim, Mohamed Ismail
• Format: Article
• Language: English
• Published: Malaysian Agricultural Research and Development Institute (MARDI) 2005
• Online Access: http://psasir.upm.edu.my/id/eprint/48938/1/Kinetics%20and%20modeling%20of%20red%20pigment%20fermentation%20by%20Monascus%20purpureus%20FTC%205391%20in%202-litre%20stirred%20tank%20fermenter%20using%20glucose%20as%20a%20carbon%20source.pdf; http://psasir.upm.edu.my/id/eprint/48938/; http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/69-2005/volume-33-no2/850-330217

The experimental data from batch fermentation were analysed to form a kinetic model of the process. The unstructured model, based on logistic and Leudeking- Piret equations, was suitable to describe growth, substrate consumption and red pigment production by Monascus purpureus FTC 5391. The maximum specific growth rate (μmax) of 0.055/h and 0.065/h were obtained from simulated modelling of M. purpureus FTC 5391 during growth in shake flask and 2-litre stirred tank fermenter, respectively. The maximum red pigment, Pmax and cell concentrations, Xmax obtained in batch fermentation using 2-litre stirred tank fermenter (20.63 UA500 and 13.2 g/litre) and using shake flask (9.26 UA500 and 11.425 g/litre) with overall productivity (P) was 0.122 UA500/h and 0.055 UA500/h, respectively. The production of red-pigment by M. purpureus FTC 5391 appeared to be a non-growth associated process; whereby rapid red-pigment production occurred during non-growth phase after the depletion of glucose in the medium.