Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus

• Main Authors: Mohd Danial, Azlina, Long, Kamariah, Mohd Ghazali, Hasanah
• Format: Article
• Language: English
• Published: Malaysian Agricultural Research and Development Institute (MARDI) 2004
• Online Access: http://psasir.upm.edu.my/id/eprint/48935/1/Improvement%20of%20the%20specific%20activity%20of%20the%20extracted%20mycelium-bound%20lipase%20of%20Aspergillus%20flavus.pdf; http://psasir.upm.edu.my/id/eprint/48935/; http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/75-2004/volume-32-no2/705-320213

Specific activity of lipase extracted from the mycelium of Aspergillus flavus, obtained from 3-day-old culture, increased nearly two times higher than from its culture supernatant. Different types of parameters were tested to optimise the extraction of mycelium-bound lipase. Highest specific activity of A. flavus lipase was obtained at 30 °C for 30 min with the agitation rate of 200 rev/min. Tris-HCl buffer (0.05 M) at pH 8.2 was the best extractant followed by Triton X-100 (0.1%), EDTA (0.01 M), NaCl (1 M) and Tween-80 (0.01%). The optimised conditions developed for the extraction of mycelium-bound lipase improved the purity of this lipase by 2.9 times higher than the control.