miR-205 in situ expression and localization in head and neck tumors - a tissue array study
Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation or translational inhibition. These molecules are known to play essential roles in many biological and physiological processes. miR-205 may be differentially expressed in head and neck cancers; however, th...
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Asian Pacific Organization for Cancer Prevention
2015
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Online Access: | http://psasir.upm.edu.my/id/eprint/45489/1/TUMOR.pdf http://psasir.upm.edu.my/id/eprint/45489/ https://pubmed.ncbi.nlm.nih.gov/25422181/ |
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my.upm.eprints.454892021-01-23T22:27:33Z http://psasir.upm.edu.my/id/eprint/45489/ miR-205 in situ expression and localization in head and neck tumors - a tissue array study Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation or translational inhibition. These molecules are known to play essential roles in many biological and physiological processes. miR-205 may be differentially expressed in head and neck cancers; however, there are conflicting data and localization of expression has yet to be determined. Materials and Methods: miR-205 expression was investigated in 48 cases of inflammatory, benign and malignant tumor tissue array of the neck, oronasopharynx,larynx and salivary glands by Locked Nucleic Acid in situ hybridization (LNA-ISH) technology. Results: miR-205 expression was significantly differentially expressed across all of the inflammatory, benign and malignant tumor tissues of the neck. A significant increase in miR-205 staining intensity (p<0.05) was observed from inflammation to benign and malignant tumors in head and neck tissue array, suggesting that miR-205 could be a biomarker to differentiate between cancer and non-cancer tissues. Conclusions: LNA-ISH revealed that miR-205 exhibited significant differential cytoplasmic and nuclear staining among inflammation, benign and malignant tumors of head and neck. miR-205 was not only exclusively expressed in squamous epithelial malignancy. This study offers information and a basis for a comprehensive study of the role of miR-205 that may be useful as a biomarker and/or therapeutic target in head and neck tumors. Asian Pacific Organization for Cancer Prevention 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45489/1/TUMOR.pdf Cheah, Yoke Kqueen and Ab Mutalib, Nurul Syakima and Lee, Learn Han (2015) miR-205 in situ expression and localization in head and neck tumors - a tissue array study. Asian Pacific journal of cancer prevention, 15 (21). pp. 9071-9075. ISSN 1513-7368 https://pubmed.ncbi.nlm.nih.gov/25422181/ 10.7314/apjcp.2014.15.21.9071 |
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Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation or translational inhibition. These molecules are known to play essential roles in many biological and physiological processes. miR-205 may be differentially expressed in head and neck cancers; however, there are conflicting data and localization of expression has yet to be determined. Materials and Methods: miR-205 expression was investigated in 48 cases of inflammatory, benign and malignant tumor tissue array of the neck, oronasopharynx,larynx and salivary glands by Locked Nucleic Acid in situ hybridization (LNA-ISH) technology. Results: miR-205 expression was significantly differentially expressed across all of the inflammatory, benign and malignant tumor tissues of the neck. A significant increase in miR-205 staining intensity (p<0.05) was observed from inflammation to benign and malignant tumors in head and neck tissue array, suggesting that miR-205 could be a biomarker to differentiate between cancer and non-cancer tissues. Conclusions: LNA-ISH revealed that miR-205 exhibited significant differential cytoplasmic and nuclear staining among inflammation, benign and malignant tumors of head and neck. miR-205 was not only exclusively expressed in squamous epithelial malignancy. This study offers information and a basis for a comprehensive study of the role of miR-205 that may be useful as a biomarker and/or therapeutic target in head and neck tumors. |
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Article |
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Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han |
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Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
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Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han |
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Cheah, Yoke Kqueen |
title |
miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
title_short |
miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
title_full |
miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
title_fullStr |
miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
title_full_unstemmed |
miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
title_sort |
mir-205 in situ expression and localization in head and neck tumors - a tissue array study |
publisher |
Asian Pacific Organization for Cancer Prevention |
publishDate |
2015 |
url |
http://psasir.upm.edu.my/id/eprint/45489/1/TUMOR.pdf http://psasir.upm.edu.my/id/eprint/45489/ https://pubmed.ncbi.nlm.nih.gov/25422181/ |
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