Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)

• Main Authors: Uda, Muhammad Nur Aiman, Che Man, Hasfalina, Abd Aziz, Samsuzana, Ishak, Zamri, Bunawan, Siti Noraini, Wahid, Nur Sabrina, Hashim, Uda
• Format: Article
• Language: English
• Published: Elsevier 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/43204/1/Comparison%20Study%20of%20Two%20Different%20Isolation%20and%20Purification.pdf; http://psasir.upm.edu.my/id/eprint/43204/; http://www.sciencedirect.com/science/article/pii/S2210784314000175

This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed with 5% Cellulases. Viral particles from both methods were examined by transmission electron microscopy (TEM) and rod-shaped with rounded ends was only seen on samples that were extracted with method 2. The estimated purity and concentration of method 2 is higher compare to method 1 which are 1.362 and 1.608 mg/ml and 0.958 and 0.299 mg/ml respectively.