Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife

Herpesvirus has been commonly isolated from captive wildlife in animal sanctuaries. Other then identification of the virus, attempts to trace the origin of the virus were initiated based on DNA restriction enzymes (RE) analysis. Two isolates from gaurs (UPMV4/05, UPMV5/05) and an isolate from a Mala...

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Main Authors: Allaudin, Zeenathul Nazariah, A. P., Zanariah, Mohd Lila, Mohd Azmi
Format: Article
Language:English
Published: Veterinary Association Malaysia 2010
Online Access:http://psasir.upm.edu.my/id/eprint/42436/1/3.pdf
http://psasir.upm.edu.my/id/eprint/42436/
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spelling my.upm.eprints.424362016-04-13T05:27:24Z http://psasir.upm.edu.my/id/eprint/42436/ Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife Allaudin, Zeenathul Nazariah A. P., Zanariah Mohd Lila, Mohd Azmi Herpesvirus has been commonly isolated from captive wildlife in animal sanctuaries. Other then identification of the virus, attempts to trace the origin of the virus were initiated based on DNA restriction enzymes (RE) analysis. Two isolates from gaurs (UPMV4/05, UPMV5/05) and an isolate from a Malayan sun bear (UPMV19/05) were examined for a homologous relationship among them. As expected, the DNA from UPMV4/05 and UPMV5/05 showed close RE similarity in comparison to UPMV19/05. The RE patterns from this herpesvirus isolates indicate a possible evolution from the same origin, bovine herpes virus (BHV). The present study showed the DNA of isolates UPMV4/05, UPMV5/05 and UPM19/05 to have an average molecular weight 0f 112x10⁶ Dalton. Veterinary Association Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/42436/1/3.pdf Allaudin, Zeenathul Nazariah and A. P., Zanariah and Mohd Lila, Mohd Azmi (2010) Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife. Jurnal Veterinar Malaysia, 22 (1 & 2). pp. 13-17. ISSN 9128-2506
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Herpesvirus has been commonly isolated from captive wildlife in animal sanctuaries. Other then identification of the virus, attempts to trace the origin of the virus were initiated based on DNA restriction enzymes (RE) analysis. Two isolates from gaurs (UPMV4/05, UPMV5/05) and an isolate from a Malayan sun bear (UPMV19/05) were examined for a homologous relationship among them. As expected, the DNA from UPMV4/05 and UPMV5/05 showed close RE similarity in comparison to UPMV19/05. The RE patterns from this herpesvirus isolates indicate a possible evolution from the same origin, bovine herpes virus (BHV). The present study showed the DNA of isolates UPMV4/05, UPMV5/05 and UPM19/05 to have an average molecular weight 0f 112x10⁶ Dalton.
format Article
author Allaudin, Zeenathul Nazariah
A. P., Zanariah
Mohd Lila, Mohd Azmi
spellingShingle Allaudin, Zeenathul Nazariah
A. P., Zanariah
Mohd Lila, Mohd Azmi
Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife
author_facet Allaudin, Zeenathul Nazariah
A. P., Zanariah
Mohd Lila, Mohd Azmi
author_sort Allaudin, Zeenathul Nazariah
title Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife
title_short Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife
title_full Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife
title_fullStr Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife
title_full_unstemmed Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife
title_sort comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife
publisher Veterinary Association Malaysia
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/42436/1/3.pdf
http://psasir.upm.edu.my/id/eprint/42436/
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