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Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates

The amino acid sequence of the fusion (F) protein of Newcastle disease virus (NDV) is of particular interest as its virulence is highly dependent on susceptibility to proteolytic cleavage. The F protein precursor, F0' can beproteolytically cleaved to form disulfide-linked F, and F2 chains. The...

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Main Authors: Ramanujam, Priadarishni, Tan, Wen Siang, Nathan, Sheila, Yusoff, Khatijah
Format: Article
Language:English
Published: Veterinary Association Malaysia 2004
Online Access:http://psasir.upm.edu.my/id/eprint/41473/1/0001.pdf
http://psasir.upm.edu.my/id/eprint/41473/
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spelling my.upm.eprints.414732015-12-21T07:52:25Z http://psasir.upm.edu.my/id/eprint/41473/ Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates Ramanujam, Priadarishni Tan, Wen Siang Nathan, Sheila Yusoff, Khatijah The amino acid sequence of the fusion (F) protein of Newcastle disease virus (NDV) is of particular interest as its virulence is highly dependent on susceptibility to proteolytic cleavage. The F protein precursor, F0' can beproteolytically cleaved to form disulfide-linked F, and F2 chains. The Fa cleavage is a prerequisite for producing infectious particles whereby the nature of the cleavage site correlates with virulence of the virus. Inthis study we determined the F protein gene cleavage site nucleotide sequence ofNDV local isolates 2641191 P2, 5953/89 P3, 1266/89 P3 and 8820/92 P3. Their sequences were also compared with other NDV strains and isolates, such as AF2240, OOIIKS, 011TM, 01lC, 3410/92 P2, 6385/90 P3, V4 Que and F that had previously been determined. The deduced amino acid sequence of this site revealed the pathogenecity of these strains whereby 2641191 P2 and 5953/89 P3 were classified as virulent isolates whilst 1266/89 P3 and 8820/92 P3 were avirulent isolates. Veterinary Association Malaysia 2004 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41473/1/0001.pdf Ramanujam, Priadarishni and Tan, Wen Siang and Nathan, Sheila and Yusoff, Khatijah (2004) Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates. Jurnal Veterinar Malaysia, 16 (1&2). pp. 9-13. ISSN 9128-2506
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The amino acid sequence of the fusion (F) protein of Newcastle disease virus (NDV) is of particular interest as its virulence is highly dependent on susceptibility to proteolytic cleavage. The F protein precursor, F0' can beproteolytically cleaved to form disulfide-linked F, and F2 chains. The Fa cleavage is a prerequisite for producing infectious particles whereby the nature of the cleavage site correlates with virulence of the virus. Inthis study we determined the F protein gene cleavage site nucleotide sequence ofNDV local isolates 2641191 P2, 5953/89 P3, 1266/89 P3 and 8820/92 P3. Their sequences were also compared with other NDV strains and isolates, such as AF2240, OOIIKS, 011TM, 01lC, 3410/92 P2, 6385/90 P3, V4 Que and F that had previously been determined. The deduced amino acid sequence of this site revealed the pathogenecity of these strains whereby 2641191 P2 and 5953/89 P3 were classified as virulent isolates whilst 1266/89 P3 and 8820/92 P3 were avirulent isolates.
format Article
author Ramanujam, Priadarishni
Tan, Wen Siang
Nathan, Sheila
Yusoff, Khatijah
spellingShingle Ramanujam, Priadarishni
Tan, Wen Siang
Nathan, Sheila
Yusoff, Khatijah
Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
author_facet Ramanujam, Priadarishni
Tan, Wen Siang
Nathan, Sheila
Yusoff, Khatijah
author_sort Ramanujam, Priadarishni
title Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
title_short Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
title_full Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
title_fullStr Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
title_full_unstemmed Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
title_sort sequence analysis of the fusion (f) protein cleavage site of four newcastle disease virus isolates
publisher Veterinary Association Malaysia
publishDate 2004
url http://psasir.upm.edu.my/id/eprint/41473/1/0001.pdf
http://psasir.upm.edu.my/id/eprint/41473/
_version_ 1643833007996403712
score 13.160551

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