Optimization of multiplex PCR conditions for rapid detection of Escherichia coli O157:H7 virulence genes

• Main Authors: Ranjit Singh, Jeshveen Singh, Chai, Lay Ching, Pui, Chai Fung, Radu, Son
• Format: Article
• Language: English
• Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2012
• Online Access: http://psasir.upm.edu.my/id/eprint/41042/1/Optimization%20of%20multiplex%20PCR%20conditions%20for%20rapid%20detection%20of%20Escherichia%20coli%20O157H7%20virulence%20genes.pdf; http://psasir.upm.edu.my/id/eprint/41042/; http://www.ifrj.upm.edu.my/19%20%2802%29%202012/%2812%29IFRJ-2012%20Jeshveen.pdf

The main source of E. coli 0157:H7 is cattle, but recent studies showed high percentage of outbreaks contributed by contaminated water. The occurrence of E. coli O157:H7 in environmental water samples poses a potential threat to human health. The aim of this study was to establish a protocol for the...