Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism

• Main Authors: Chai, Lay Ching, Abdullah, Fatimah Corazon, Mat Sout, Norhisyam, Alias, Rozila, Abu Samah, Nadzirah, Lee, Natasha Hai Yen
• Format: Article
• Language: English
• Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/40902/1/15%20IFRJ-2008-140%20Chai%20Malaysia%20FINAL.pdf; http://psasir.upm.edu.my/id/eprint/40902/; http://www.ifrj.upm.edu.my/16%20%281%29%202009/15%20IFRJ-2008-140%20Chai%20Malaysia%20FINAL.pdf

The objective of the present study was to develop a rapid, reliable and yet inexpensive protocol for genomic DNA extraction from frozen and ethanol-preserved Asian green-lipped mussels for random amplified microsatelite (RAM) analysis. The procedure comprised of three major steps: (1) Tissue degrada...