Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates

• Main Authors: Chang, Sui Kiat, Hamajima, Hiroshi, Ismail, Amin, Yanagita, Teruyoshi, Mohd Esa, Norhaizan, Baharuldin, Mohamad Taufik Hidayat
• Format: Article
• Language: English
• Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/40781/1/9%20IFRJ%2021%20%2803%29%202014%20Chang%20713.pdf; http://psasir.upm.edu.my/id/eprint/40781/; http://www.ifrj.upm.edu.my/21%20%2803%29%202014/9%20IFRJ%2021%20%2803%29%202014%20Chang%20713.pdf

This study was conducted to ascertain the cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates (OPKHs) produced from its protein isolate. A modified microplate titer WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay was used to investigate the cytotoxicity of hydrolysates produced from protease and pepsin-pancreatin hydrolysis at various concentrations (0.1, 1, 10, 100 μg/ml and 1 mg/ml) using HepG2 cell model. Additionally, peptide stimulation test using OPKHs at 1 mg/ml was carried out to investigate whether OPKHs could serve as growth factor for HepG2 cells other than affecting its viability. As a result, oleic acid appeared to normalize the WST-1 readings of HepG2 cells treated with both hydrolysates at 1 mg/ml. The presence of amino acids in OPKHs could stimulate the growth and prolongs the viability of HepG2 cells. Both OPKHs were non-cytotoxic to HepG2 cells at all tested concentrations even at high concentrations. This study indicated that pepsin-pancreatin and protease hydrolysates produced from oil palm kernel protein were non-cytotoxic on HepG2 cells.