Repeated fed-batch cultivation of nitrogen-fixing bacterium, Bacillus sphaericus UPMB10, using glycerol as the carbon source

• Main Authors: Ariff, Arbakariya, Ooi, Tze Chean, Mohd Saud, Halimi, Shamsuddin, Zulkifli
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia Press 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/40581/1/Repeated%20Fed-Batch%20Cultivation%20of%20Nitrogen-Fixing%20Bacterium%2C%20Bacillus%20sphaericus%20UPMB10%2C%20Using%20Glycerol%20as%20the%20Carbon%20Source.pdf; http://psasir.upm.edu.my/id/eprint/40581/; http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JST%20Vol.%2018%20%282%29%20Jul.%202010/15%20Pg%20365-375.pdf

The exponential fed-batch cultivation of Bacillus sphaericus UPMB10 in 2 l stirred tank fermenter was performed by feeding the initial batch culture with 14 g l-1 of glycerol according to the algorithm aimed at controlling the specific growth rate (µ) of the bacterium. Very high viable cell count (1...