Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds

• Main Authors: Guo, Hui Fang, Abd Hamid @ Abdul Razak, Roslida, Mohd Ali, Razana, Chang, Sui Kiat, Rahman, Mohammed Habibur, Zainal, Zaida, Khaza'ai, Huzwah
• Format: Article
• Language: English
• Published: MDPI 2020
• Online Access: http://psasir.upm.edu.my/id/eprint/38172/1/38172.pdf; http://psasir.upm.edu.my/id/eprint/38172/; https://www.mdpi.com/2076-3921/9/2/130

Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided into six groups of six each and were: untreated control, treated with Silverdin® cream, base cream, base cream with c% EGF, base cream with 3% TRF or base cream with c% EGF and 3% TRF, respectively. Creams were applied once daily for 21 consecutive days. Six animals from each group were sacrificed using anaesthetic overdose on the third, seventh, 11th, 14th and 21st day post-burn. Skin tissues with the wound to be examined were excised for macroscopic and microscopic evaluation and biochemical analyses. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. However, no effects on the number of adipose cells in the healing process were recorded. In addition, lipid peroxidation and nitrite production were found to be reduced post-burn, reducing oxidative stress. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters.