Comparison of cryopreservative effect of different levels of omega-3 egg-yolk in citrate extender on the quality of goat spermatozoa

• Main Authors: Yimer, Nurhusien, A. H., Noraisyah, Yusoff, Rosnina, Haron, Abd Wahid, Kazhal, Sarsaifi, A. M., Hafizal
• Format: Article
• Language: English
• Published: Faculty of Veterinary Science, University of Agriculture, Faisalabad 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/36838/1/Comparison%20of%20cryopreservative%20effect%20of%20different%20levels%20of%20omega.pdf; http://psasir.upm.edu.my/id/eprint/36838/; http://www.pvj.com.pk/pdf-files/34_3/347-350.pdf

The objective of the present study was to compare quality of chilled and frozenthawed goat semen processed in citrate extender containing 3 different levels (2.5, 5 and 10%) of omega-3 egg-yolk (EY). Ejaculates were collected from five adult goats using artificial vagina. Quality of fresh semen, processed semen after 3 hrs of chilling and 24 hrs of freezing was assessed based on live sperm %age, abnormality (determined by eosin-nigrosin stain) and general and progressive motility (evaluated by CEROS computer assisted semen analyzer). The result showed a significant (P<0.05) decrease in post-chilled live sperm %age from the fresh sample for extenders using 2.5 and 5% EY but not for the 10%. Moreover, a significantly lower percentage general and progressive motility was recorded using the 2.5% EY compared to the others that showed post-chilled sperm motility non-significantly different from the fresh sample. After chilling, 5% EY showed significantly lower percent sperm abnormalities compared to others. However, the abnormalities increased after freezing to a level non-significantly different from the 10% EY that sustained to demonstrate higher live sperm %age and motility than both 2.5 and 5% EY. An overall increase in post-thawed live sperm %age, general and progressive motility was observed with increase in concentration of EY added. Thus, though the difference with the 5% EY is in magnitude, the 10% omega-3 EY in citrate extender is preferred compared to 2.5% for superior post-thawed goat semen quality, extended without washing seminal plasma.