Application of PCR-ELISA in molecular diagnosis

• Main Authors: Sue, Mei Jean, Yeap, Swee Keong, Omar, Abdul Rahman, Tan, Sheau Wei
• Format: Article
• Language: English
• Published: Hindawi Publishing Corporation 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/36517/1/Application%20of%20PCR.pdf; http://psasir.upm.edu.my/id/eprint/36517/; http://www.hindawi.com/journals/bmri/2014/653014/

Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.