A higher sensitivity and efficiency of common primer multiplex PCR assay in identification of meat origin using NADH dehydrogenase subunit 4 gene

• Main Authors: Hanapi, Ummi Kalthum, Mohd Desa, Mohd Nasir, Ismail, Amin, Mustafa, Shuhaimi
• Format: Article
• Language: English
• Published: Springer 2015
• Online Access: http://psasir.upm.edu.my/id/eprint/36170/1/A%20higher%20sensitivity%20and%20efficiency%20of%20common%20primer%20multiplex%20PCR%20assay%20in%20identification%20of%20meat%20origin%20using%20NADH%20dehydrogenase%20subunit%204%20gene.pdf; http://psasir.upm.edu.my/id/eprint/36170/; http://link.springer.com/article/10.1007%2Fs13197-014-1459-7

A Common Primer Multiplex PCR (CP-M-PCR) was developed to detect meat origin of four groups of animal (pig, ruminant, avian and rabbit). This method demonstrated higher sensitivity and efficiency than the conventional multiplex PCR. In this approach, a common forward primer was designed in the 5′ en...