Vegetative compatibility of Fusarium oxysporum isolated from various plants
Fusarium oxysporum is common plant pathogen and is classified into divisions called formae speciales according to host specificity. Thirty isolates of F. oxysporum was isolated from various plant samples from various locations in Malaysia. Chlorate-resistant sectors (CRSs) were generated and phenoty...
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| Main Authors: | , |
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| Format: | Article |
| Published: |
Ecological Association of Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35979/ http://www.mybioacta.com/index.php/article-in-press/58-030116-23 |
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| Summary: | Fusarium oxysporum is common plant pathogen and is classified into divisions called formae speciales according to host specificity. Thirty isolates of F. oxysporum was isolated from various plant samples from various locations in Malaysia. Chlorate-resistant sectors (CRSs) were generated and phenotyped on the phenotyping media that contain different nitrogen sources. Twenty-seven of F. oxysporum isolates were identified as heterokaryon self-compatible (HSC) while three isolates as heterokaryon self-incompatible (HSI) after repeated attempts. Those isolates that formed heterokaryon when the nit mutants merged with different isolates were classified into same vegetative compatibility group (VCG) while those unable were classified into different VCGs. Twenty VCGs were obtained, four VCGs contained more than one isolate and another 16 VCGs contained a single isolate in each group. The percentage of VCG diversity of F. oxysporum was 74%. F. oxysporum isolated from various plants samples in Malaysia were genetically diverse based on their multiple VCGs. |
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