Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells
Introduction: Mesenchymal stem cells (MSCs) hold a great therapeutic potential for regenerative medicine and tissue engineering due to inherent immunomodulatory and reparative properties. Hence, it necessitates a readily available supplyof MSCs to meet the clinical demands adequately. Although, a hu...
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2016
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my.upm.eprints.352522016-10-12T04:20:48Z http://psasir.upm.edu.my/id/eprint/35252/ Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells Vellasamy, Shalini Vidyadaran, Sharmili George, Elizabeth Ramasamy, Rajesh Introduction: Mesenchymal stem cells (MSCs) hold a great therapeutic potential for regenerative medicine and tissue engineering due to inherent immunomodulatory and reparative properties. Hence, it necessitates a readily available supplyof MSCs to meet the clinical demands adequately. Although, a human placenta can produce MSCs, the in vitro culture-mediated cellular senescence often affect the quality of cell product. Thus, the current study has explored the feasibility of basic fibroblast growth factor (bFGF) to enhance the growth of placenta-derived MSCs (PLC-MSCs). Methods: The basic fibroblast growth factor (bFGF) was supplemented to optimise the growth of MSCs. The effects of bFGF on morphology, growth kinetics and cytokine secretion of PLC-MSCs were assessed. Results: The bFGF supplementation increased the proliferation of PLC-MSCs in a dose-dependent manner and 40 ng/ml showed a high trophism effect on PLC-MSC’s growth. In the presence of bFGF, PLC-MSCs acquired a small and well-defined morphology that reflect an active proliferative status. BFGF has induced PLC-MSCs to achieve a shorter doubling time (45 hrs) as compared to the non-supplemented PLC-MSCs culture (81 hrs). Furthermore, bFGF impelled PLC-MSCs into cell cycle machinery where a substantial fraction of cells was driven to S and G2/M phases. Amongst, 36 screened cytokines, bFGF had only altered the secretion of IL-8, IL-6, TNFR1, MMP3 and VEGF. Conclusion: The present study showed that bFGF supplementation promotes the growth of PLC-MSCs without significantly deviating from the standard criteria of MSCs. Thus, bFGF could be considered as a potential mitogen to facilitate the large-scale production of PLC-MSCs. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35252/1/FKUSK1_ARTICLE_6.pdf Vellasamy, Shalini and Vidyadaran, Sharmili and George, Elizabeth and Ramasamy, Rajesh (2016) Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells. Malaysian Journal of Medicine and Health Sciences, 12 (1). pp. 49-59. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_ARTICLE_6.pdf |
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Introduction: Mesenchymal stem cells (MSCs) hold a great therapeutic potential for regenerative medicine and tissue engineering due to inherent immunomodulatory and reparative properties. Hence, it necessitates a readily available supplyof MSCs to meet the clinical demands adequately. Although, a human placenta can produce MSCs, the in vitro culture-mediated cellular senescence often affect the quality of cell product. Thus, the current study has explored the feasibility of basic fibroblast growth factor (bFGF) to enhance the growth of placenta-derived MSCs (PLC-MSCs). Methods: The basic fibroblast growth factor (bFGF) was supplemented to optimise the growth of MSCs. The effects of bFGF on morphology, growth kinetics and cytokine secretion of PLC-MSCs were assessed. Results: The bFGF supplementation increased the proliferation of PLC-MSCs in a dose-dependent manner and 40 ng/ml showed a high trophism effect on PLC-MSC’s growth. In the presence of bFGF, PLC-MSCs acquired a small and well-defined morphology that reflect an active proliferative status. BFGF has induced PLC-MSCs to achieve a shorter doubling time (45 hrs) as compared to the non-supplemented PLC-MSCs culture (81 hrs). Furthermore, bFGF impelled PLC-MSCs into cell cycle machinery where a substantial fraction of cells was driven to S and G2/M phases. Amongst, 36 screened cytokines, bFGF had only altered the secretion of IL-8, IL-6, TNFR1, MMP3 and VEGF. Conclusion: The present study showed that bFGF supplementation promotes the growth of PLC-MSCs without significantly deviating from the standard criteria of MSCs. Thus, bFGF could be considered as a potential mitogen to facilitate the large-scale production of PLC-MSCs. |
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Article |
author |
Vellasamy, Shalini Vidyadaran, Sharmili George, Elizabeth Ramasamy, Rajesh |
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Vellasamy, Shalini Vidyadaran, Sharmili George, Elizabeth Ramasamy, Rajesh Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells |
author_facet |
Vellasamy, Shalini Vidyadaran, Sharmili George, Elizabeth Ramasamy, Rajesh |
author_sort |
Vellasamy, Shalini |
title |
Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells |
title_short |
Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells |
title_full |
Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells |
title_fullStr |
Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells |
title_full_unstemmed |
Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells |
title_sort |
basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells |
publisher |
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
publishDate |
2016 |
url |
http://psasir.upm.edu.my/id/eprint/35252/1/FKUSK1_ARTICLE_6.pdf http://psasir.upm.edu.my/id/eprint/35252/ http://www.medic.upm.edu.my/dokumen/FKUSK1_ARTICLE_6.pdf |
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