Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435

• Main Authors: Abdu, Hamisu, Ahmad @ Amat, Faujan, Basri, Mahiran, Ismail, Intan Safinar, Abdul Rahman, Mohd Basyaruddin
• Format: Article
• Language: English
• Published: A & V Publications 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/34770/1/Optimization%20of%20enzymatic%20synthesis%20of%203-O-%CE%B2-D-glucopyranoside%20betulinic%20acid%20by%20Novozyme-435.pdf; http://psasir.upm.edu.my/id/eprint/34770/; http://ajrconline.org/AbstractView.aspx?PID=2014-7-7-6

The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69 % and the predicted value was 89.05 %.