Optimal conditions for hepatitis B core antigen production in shaked flask fermentation

• Main Authors: Tey, Beng Ti, Yong, Kok Hoe, Ong, Hong Puay, Ling, Tau Chuan, Ong, Swee Tin, Tan, Yan Peng, Ariff, Arbakariya, Tan, Wen Siang
• Format: Article
• Language: English
• Published: Korean Society for Biotechnology and Bioengineering 2004
• Online Access: http://psasir.upm.edu.my/id/eprint/34502/1/Optimal%20conditions%20for%20hepatitis%20B%20core%20antigen%20production%20in%20shaked%20flask%20fermentation.pdf; http://psasir.upm.edu.my/id/eprint/34502/; http://link.springer.com/article/10.1007/BF02933060?view=classic

The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and 40°C) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present study. The highest growth rate is achieved at PH 7, 37°C and at a rotational speed of 250 rpm which is 0.927 h−1. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a 123% different between the highest growth rate (0.927 h−1) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and 40°C. The yield of protein at pH 7 is 154% higher compared to the lowest yield achieved at pH 5. There is about 28% different of the protein yield for theE. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at 40°C is 38% higher compared to the lowest yield achieved, at 30°C.