Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization
Silicon (Si) is the second most abundant element in soil after oxygen. It is not an essential element for plant growth and formation but plays an important role in increasing plant tolerance towards different kinds of abiotic and biotic stresses. The molecular mechanism of Si absorption and accumula...
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Hindawi Publishing Corporation
2014
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my.upm.eprints.342872017-03-31T09:48:34Z http://psasir.upm.edu.my/id/eprint/34287/ Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization Sahebi, Mahbod Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Yusop, Mohd Rafii Azizi, Parisa Nejat, Naghmeh Idris, Abu Seman Silicon (Si) is the second most abundant element in soil after oxygen. It is not an essential element for plant growth and formation but plays an important role in increasing plant tolerance towards different kinds of abiotic and biotic stresses. The molecular mechanism of Si absorption and accumulation may differ between plants, such as monocotyledons and dicotyledons. Silicon absorption and accumulation in mangrove plants are affected indirectly by some proteins rich in serine and proline amino acids. The expression level of the genes responsible for Si absorption varies in different parts of plants. In this study, Si is mainly observed in the epidermal roots’ cell walls of mangrove plants compared to other parts. The present work was carried out to discover further information on Si stress responsive genes in Rhizophora apiculata, using the suppression subtractive hybridization technique. To construct the cDNA library, two-month-old seedlings were exposed to 0.5, 1, and 1.5 mM SiO2 for 15 hrs and for 1 to 6 days resulting in a total of 360 high quality ESTs gained. Further examination by RT-PCR and real-time qRT-PCR showed the expression of a candidate gene of serine-rich protein. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34287/7/ITA%202014%201RR.pdf Sahebi, Mahbod and Musa, Mohamed Hanafi and Abdullah, Siti Nor Akmar and Yusop, Mohd Rafii and Azizi, Parisa and Nejat, Naghmeh and Idris, Abu Seman (2014) Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization. BioMed Research International , 2014. art. no. 971985. pp. 1-11. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2014/971985/abs/ 10.1155/2014/971985 |
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Silicon (Si) is the second most abundant element in soil after oxygen. It is not an essential element for plant growth and formation but plays an important role in increasing plant tolerance towards different kinds of abiotic and biotic stresses. The molecular mechanism of Si absorption and accumulation may differ between plants, such as monocotyledons and dicotyledons. Silicon absorption and accumulation in mangrove plants are affected indirectly by some proteins rich in serine and proline amino acids. The expression level of the genes responsible for Si absorption varies in different parts of plants. In this study, Si is mainly observed in the epidermal roots’ cell walls of mangrove plants compared to other parts. The present work was carried out to discover further information on Si stress responsive genes in Rhizophora apiculata, using the suppression subtractive hybridization technique. To construct the cDNA library, two-month-old seedlings were exposed to 0.5, 1, and 1.5 mM SiO2 for 15 hrs and for 1 to 6 days resulting in a total of 360 high quality ESTs gained. Further examination by RT-PCR and real-time qRT-PCR showed the expression of a candidate gene of serine-rich protein. |
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Sahebi, Mahbod Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Yusop, Mohd Rafii Azizi, Parisa Nejat, Naghmeh Idris, Abu Seman |
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Sahebi, Mahbod Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Yusop, Mohd Rafii Azizi, Parisa Nejat, Naghmeh Idris, Abu Seman Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization |
author_facet |
Sahebi, Mahbod Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Yusop, Mohd Rafii Azizi, Parisa Nejat, Naghmeh Idris, Abu Seman |
author_sort |
Sahebi, Mahbod |
title |
Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization |
title_short |
Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization |
title_full |
Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization |
title_fullStr |
Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization |
title_full_unstemmed |
Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization |
title_sort |
isolation and expression analysis of novel silicon absorption gene from roots of mangrove (rhizophora apiculata) via suppression subtractive hybridization |
publisher |
Hindawi Publishing Corporation |
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2014 |
url |
http://psasir.upm.edu.my/id/eprint/34287/7/ITA%202014%201RR.pdf http://psasir.upm.edu.my/id/eprint/34287/ http://www.hindawi.com/journals/bmri/2014/971985/abs/ |
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