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A process for producing lipase

A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O,...

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Main Author:	Raja Abdul Rahman, Raja Noor Zaliha
Format:	Patent
Published:	2013
Online Access:	http://psasir.upm.edu.my/id/eprint/33645/
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id	my.upm.eprints.33645
record_format	eprints
spelling	my.upm.eprints.336452016-09-27T01:19:01Z http://psasir.upm.edu.my/id/eprint/33645/ A process for producing lipase Raja Abdul Rahman, Raja Noor Zaliha A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside. 2013-05-20 Patent NonPeerReviewed Raja Noor Zaliha Raja Abd. Rahman (2013) A process for producing lipase. PI2013700818.
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside.
format	Patent
author	Raja Abdul Rahman, Raja Noor Zaliha
spellingShingle	Raja Abdul Rahman, Raja Noor Zaliha A process for producing lipase
author_facet	Raja Abdul Rahman, Raja Noor Zaliha
author_sort	Raja Abdul Rahman, Raja Noor Zaliha
title	A process for producing lipase
title_short	A process for producing lipase
title_full	A process for producing lipase
title_fullStr	A process for producing lipase
title_full_unstemmed	A process for producing lipase
title_sort	process for producing lipase
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/33645/
_version_	1643830930883739648
score	13.214268

A process for producing lipase

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